Touchstone Diabetes Center, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX 75390-8542, USA.
Cell Metab. 2018 Jan 9;27(1):252-262.e3. doi: 10.1016/j.cmet.2017.12.004.
[F]Fluorodeoxyglucose-PET/CT (F-FDG-PET/CT) imaging has been invaluable for visualizing metabolically active adipose tissues in humans with potential anti-diabetic and anti-obesity effects. To explore whether mice display human-like fat depots in anatomically comparable regions, we mapped fat depots using glucose or fatty acid imaging tracers, such as F-FDG through PET/CT or [I]-β-methyl-p-iodophenyl-pentadecanoic acid with SPECT/CT imaging, to analogous depots in mice. Using this type of image analysis with both probes, we define a large number of additional areas of high metabolic activity corresponding to novel fat pads. Histological and gene expression analyses validate these regions as bona fide fat pads. Our findings indicate that fat depots of rodents show a high degree of topological similarity to those of humans. Studies involving both glucose and lipid tracers indicate differential preferences for these substrates in different depots and also suggest that fatty acid-based visualized approaches may reveal additional brown adipose tissue and beige depots in humans.
[F]氟脱氧葡萄糖正电子发射断层扫描/计算机断层扫描（F-FDG-PET/CT）成像在可视化人类具有潜在抗糖尿病和抗肥胖作用的代谢活跃脂肪组织方面具有重要价值。为了探索小鼠是否在解剖学上可比的区域显示出类似人类的脂肪沉积，我们使用葡萄糖或脂肪酸成像示踪剂（如通过 PET/CT 的 F-FDG 或 SPECT/CT 成像的[I]-β-甲基-p-碘代苯戊酸）来映射脂肪沉积，以在小鼠中对应类似的沉积。使用这两种探针的这种类型的图像分析，我们定义了大量与新脂肪垫相对应的高代谢活性区域。组织学和基因表达分析验证了这些区域是真正的脂肪垫。我们的研究结果表明，啮齿动物的脂肪沉积与人类的脂肪沉积具有高度的拓扑相似性。涉及葡萄糖和脂质示踪剂的研究表明，不同的沉积对这些底物具有不同的偏好，并且还表明基于脂肪酸的可视化方法可能会在人类中揭示更多的棕色脂肪组织和米色脂肪组织。
