Chen Yu-Jen, Liao Pei-Xin, Kuo Wen-Hung, Chen Ching-Yi, Ding Shih-Torng, Wang Mei-Hui
Institute of Biotechnology and Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan.
Department of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.
Methods Mol Biol. 2023;2662:135-145. doi: 10.1007/978-1-0716-3167-6_12.
In response to cold induction, brown adipose tissues (BAT) and emerged brown-like adipocytes (beige adipocytes) in subcutaneous white adipose tissues (WAT browning/beiging) are activated. Thermogenesis is increased during glucose and fatty acid uptake and metabolism in adult humans and mice. This activation of BAT or WAT beiging to generate heat helps to counteract diet-induced obesity. This protocol applies the glucose analog radiotracer F-fluorodeoxyglucose (FDG), coupled with positron emission tomography and computed tomography (PET/CT) scanning to evaluate cold-induced thermogenesis in the active BAT (interscapular region) and browned/beiged WAT (subcutaneous adipose region) in mice. The PET/CT scanning technique not only can quantify cold-induced glucose uptake in well-known BAT and beige-fat depots but also helps to visualize the anatomical location of novel uncharacterized mouse BAT and beige fat where cold-induced glucose uptake is high. Histological analysis is further employed to validate signals of delineated anatomical regions in PET/CT images as bona fide mouse BAT or beiged WAT fat depots.
在冷诱导作用下，棕色脂肪组织（BAT）以及皮下白色脂肪组织中出现的棕色样脂肪细胞（米色脂肪细胞）（白色脂肪组织褐变/米色化）会被激活。在成年人类和小鼠中，葡萄糖和脂肪酸摄取及代谢过程中的产热作用增强。BAT的这种激活或白色脂肪组织米色化以产生热量，有助于对抗饮食诱导的肥胖。本方案应用葡萄糖类似物放射性示踪剂F-氟脱氧葡萄糖（FDG），结合正电子发射断层扫描和计算机断层扫描（PET/CT）来评估小鼠活跃BAT（肩胛间区域）和褐变/米色化白色脂肪组织（皮下脂肪区域）中的冷诱导产热。PET/CT扫描技术不仅可以量化在已知的BAT和米色脂肪库中冷诱导的葡萄糖摄取，还有助于可视化新的未表征小鼠BAT和米色脂肪的解剖位置，这些部位冷诱导的葡萄糖摄取量很高。进一步采用组织学分析来验证PET/CT图像中划定的解剖区域的信号，确认为真正的小鼠BAT或米色化白色脂肪组织脂肪库。
