Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.
Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.
Sci Rep. 2018 Jan 10;8(1):390. doi: 10.1038/s41598-017-18910-w.
Three carbapenem-resistant Escherichia coli were recovered from rectal swabs of different patients in a tertiary hospital and were found carrying bla an uncommon bla variant. Genome sequences of the isolates were obtained using Illumina technology and the long-read MinION sequencer. The isolates belonged to ST405 and phylogenetic group D, a globally distributed lineage associated with antimicrobial resistance. In addition to bla , the three isolates carried 14 known resistance genes including the extended-spectrum β-lactamase gene bla . There were only 1 or 2 SNPs between the isolates, suggesting a common origin and cryptic transmission in hospital. bla was located on a 46.5-kb IncFIA self-transmissible plasmid, which may facilitate further dissemination of bla . Two copies of IS26 bracketed a 14.6-kb region containing bla and have the potential to form a composite transposon for mediating the mobilization of bla .
从一家三甲医院不同患者的直肠拭子中分离到 3 株耐碳青霉烯类大肠埃希菌，均携带 bla 型罕见 bla 变体。使用 Illumina 技术和长读长 MinION 测序仪获得分离株的基因组序列。这些分离株属于 ST405 和进化群 D，这是一种与抗微生物药物耐药性相关的全球分布谱系。除了 bla ，这 3 株分离株还携带 14 种已知的耐药基因，包括扩展谱β-内酰胺酶基因 bla 。分离株之间只有 1 或 2 个 SNP，提示在医院中存在共同的起源和隐匿传播。bla 位于一个 46.5kb 的 IncFIA 自我传播质粒上，这可能有助于 bla 的进一步传播。两个 IS26 拷贝框定了一个包含 bla 和的 14.6kb 区域，具有形成复合转座子的潜力，可介导 bla 的动员。
