Rinaldo P, Miolo G, Chiandetti L, Zacchello F, Daolio S, Traldi P
Biomed Mass Spectrom. 1985 Sep;12(9):570-6. doi: 10.1002/bms.1200120922.
Two kinds of inborn errors of metabolism, dicarboxylic aciduria and hyperoxaluria, have been studied by means of hydroxyl negative ion chemical ionization [NICI(OH-)], linked with collisionally activated decomposition experiments on the [M-H]- species of the pathognomonic organic acids. This method has led to non-controversial qualitative determinations of C4-C10 dicarboxylic acids and oxalic, glyceric and glyoxylic acids. NICI(OH-) linked with collisionally activated decomposition mass analysed ion kinetic energy spectrometry (CAD MIKES) is proposed herein for diagnostic purposes, as a valid mass spectrometric alternative to standard gas chromatographic/mass spectrometric analysis. The procedure is characterized by simplified sample treatment and by fast execution.
通过羟基负离子化学电离法[NICI(OH-)]，结合对特征有机酸的[M-H]-物种进行碰撞激活分解实验，对两种先天性代谢缺陷病——二羧酸尿症和高草酸尿症进行了研究。该方法已成功用于对C4 - C10二羧酸以及草酸、甘油酸和乙醛酸进行无争议的定性测定。本文提出将NICI(OH-)与碰撞激活分解质量分析离子动能谱法（CAD MIKES）相结合用于诊断目的，作为标准气相色谱/质谱分析的有效质谱替代方法。该方法的特点是样品处理简单且执行速度快。
