Rinaldo P, Chiandetti L, Zacchello F, Daolio S, Traldi P
Biomed Mass Spectrom. 1984 Dec;11(12):643-6. doi: 10.1002/bms.1200111209.
A novel application of Collisionally Activated Decomposition Mass analysed Ion Kinetic Energy (CAD MIKE) spectrometry to separation and positive structural identification of urinary methylmalonic acid (MMA) (the pathognomonic compound for the diagnosis of methylmalonic acidurias) is presented. CAD MIKES scans of EI ionic species at m/z 119 ([M + H]+) and m/z 101 ([M-OH]+) have been obtained from a pure standard of MMA and from crude urinary acid fractions. With reference to the procedures employed so far, the advantages of the proposed method lie in fast and simplified sample pretreatment and in a quick non-controversial response to a clinical suspicion of serious, life-threatening inherited metabolic diseases.
介绍了碰撞激活分解质量分析离子动能（CAD MIKE）光谱法在尿液甲基丙二酸（MMA）（甲基丙二酸尿症诊断的特征性化合物）分离和阳性结构鉴定中的新应用。已从MMA纯标准品和尿酸性粗馏分中获得了m/z 119（[M + H]+）和m/z 101（[M-OH]+）处EI离子物种的CAD MIKES扫描。参照迄今为止所采用的程序，该方法的优点在于快速、简化的样品预处理，以及对严重的、危及生命的遗传性代谢疾病临床怀疑的快速、无争议的反应。
