Rynjah Carey Vana, Devi Ningthoujam Neelima, Khongthaw Nasheman, Syiem Donkupar, Majaw Suktilang
Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya, 793022, India.
Department of Biochemistry, North-Eastern Hill University, Shillong, Meghalaya, 793022, India.
J Tradit Complement Med. 2017 May 25;8(1):134-140. doi: 10.1016/j.jtcme.2017.04.007. eCollection 2018 Jan.
The present study was designed to evaluate the antidiabetic potential of the aqueous leaves extract of DC. leaves using and approaches. For studies, blood glucose level was monitored at different intervals after administration of varying doses of the extract for its hypoglycemic (100-6000 mg/kg b.w.) and antihyperglycemic (250 mg/kg b.w.) effect in normoglycemic and diabetic mice. enzymatic inhibition activity was tested against α-amylase, α- and β-glucosidase and lipase. Additionally hydroxyl radical, hydrogen peroxide scavenging assay and phytochemical screening were also performed. Element analysis of the plant was studied by Atomic Absorption Spectrometry (AAS) and Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES). The plant extract showed significant hypoglycemic and antihyperglycemic effect in normoglycemic and diabetic mice. The IC values of extract for α-amylase, β-glucosidase, lipase, hydroxyl radical scavenging activity, hydrogen peroxide scavenging activity were 7.40 mg/ml, 0.30 mg/ml, 8.35 mg/ml, 3.25 mg/ml, 9.62 mg/ml respectively and the percentage of inhibition for α-glucosidase was 79.82% at 0.8 mg/ml. studies were compared with their respective standards. Elemental analysis revealed the presence of essential elements such as Mg, V, Fe, Cr, Zn, Cu, Mo, Mn, K, Ca, P and Sr which are all known to play a role in regulating blood glucose. The results demonstrate that aqueous leaves extract possess antidiabetic property in both and condition.
本研究旨在利用[具体方法1]和[具体方法2]评估DC.叶水提物的抗糖尿病潜力。对于[相关实验1]研究，在给予不同剂量提取物（降血糖剂量为100 - 6000mg/kg体重，抗高血糖剂量为250mg/kg体重）后，在不同时间间隔监测正常血糖和糖尿病小鼠的血糖水平，以观察其降血糖和抗高血糖作用。测试了提取物对α -淀粉酶、α -和β -葡萄糖苷酶以及脂肪酶的酶抑制活性。此外，还进行了羟自由基、过氧化氢清除试验以及植物化学筛选。通过原子吸收光谱法（AAS）和电感耦合等离子体原子发射光谱仪（ICP - AES）对该植物进行元素分析。该植物提取物在正常血糖和糖尿病小鼠中均显示出显著的降血糖和抗高血糖作用。提取物对α -淀粉酶、β -葡萄糖苷酶、脂肪酶、羟自由基清除活性、过氧化氢清除活性的IC值分别为7.40mg/ml、0.30mg/ml、8.35mg/ml、3.25mg/ml、9.62mg/ml，在0.8mg/ml时对α -葡萄糖苷酶的抑制率为79.82%。[相关实验1]研究与各自的标准进行了比较。元素分析表明该植物含有必需元素，如Mg、V、Fe、Cr、Zn、Cu、Mo、Mn、K、Ca、P和Sr，这些元素均已知在调节血糖中发挥作用。结果表明，DC.叶水提物在正常血糖和糖尿病状态下均具有抗糖尿病特性。
