Xu Huihui, Tie Kunyuan, Zhang Yang, Feng Xin, Cao Yuan, Han Wenyu
College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
J Pept Sci. 2018 Jan;24(1). doi: 10.1002/psc.3059.
The overuse of antibiotics has resulted in the emergence of antibiotic-resistant bacteria, which presents an urgent need for new antimicrobial agents. At present, antimicrobial peptides have attracted a great deal of attention from researchers. However, antimicrobial peptides often affect a broad range of microorganisms, including the normal flora in a host organism. In the present study, we designed a novel hybrid antimicrobial peptide, expressed the hybrid peptide, and studied its specific target. The hybrid peptide, named T-catesbeianin-1, which includes the FyuA-binding domain of pesticin and the peptide catesbeianin-1, was designed and expressed in Pichia pastoris X-33. Then, we determined the antimicrobial activity, cytotoxicity, and specific target of the peptide. T-catesbeianin-1 has strong antimicrobial activity and binds to FyuA to inhibit or kill Escherichia coli present in clinical specimens and mixed-species culture. In summary, these findings suggested that T-catesbeianin-1 might be promising and specific antibiotic agent for therapeutic application against fyuA E. coli.
抗生素的过度使用导致了抗生素耐药菌的出现，这迫切需要新的抗菌剂。目前，抗菌肽已引起研究人员的广泛关注。然而，抗菌肽通常会影响广泛的微生物，包括宿主生物体中的正常菌群。在本研究中，我们设计了一种新型杂合抗菌肽，表达了该杂合肽，并研究了其特异性靶点。这种名为T-catesbeianin-1的杂合肽，包含杀鼠菌素的FyuA结合结构域和肽catesbeianin-1，在毕赤酵母X-33中进行了设计和表达。然后，我们测定了该肽的抗菌活性、细胞毒性和特异性靶点。T-catesbeianin-1具有很强的抗菌活性，它与FyuA结合，以抑制或杀死临床标本和混合菌群培养物中存在的大肠杆菌。总之，这些发现表明T-catesbeianin-1可能是一种有前景的、针对携带fyuA的大肠杆菌的特异性抗生素治疗剂。
