Disteche C M, Tantravahi U, Gandy S, Eisenhard M, Adler D, Kunkel L M
Cytogenet Cell Genet. 1985;39(4):262-8. doi: 10.1159/000132155.
Two repetitive DNA fragments located on the mouse X chromosome are described. The fragments were isolated from a lambda phage library enriched in X-chromosomal sequences by flow sorting. Both fragments, which are repeated 20 to 50 times in the genome, were mapped to the mouse X chromosome by Southern blot hybridization to DNA from hybrid cells retaining the mouse X chromosome, by dosage analysis, and by in situ hybridization to mouse chromosomes. In mouse strain C57BL/10BK, one fragment appeared to be located only on the X chromosome, while the other fragment had homologous sequences on chromosome 11 in addition to the X chromosome. The latter fragment showed DNA variants between mouse strains, which are potentially useful for mapping. Both fragments cross-hybridized to another mouse species: Mus caroli. In this species, each fragment appeared to be located on the X chromosome, indicating that some X-chromosome repetitive sequences are partially conserved. In addition, one fragment cross-hybridized to human DNA.
本文描述了位于小鼠X染色体上的两个重复DNA片段。这些片段是从通过流式细胞分选富集X染色体序列的λ噬菌体文库中分离出来的。通过与保留小鼠X染色体的杂交细胞的DNA进行Southern印迹杂交、剂量分析以及与小鼠染色体的原位杂交，将这两个在基因组中重复20至50次的片段定位到小鼠X染色体上。在小鼠品系C57BL/10BK中，一个片段似乎仅位于X染色体上，而另一个片段除了X染色体外，在11号染色体上也有同源序列。后一个片段在小鼠品系之间表现出DNA变异，这可能有助于基因定位。这两个片段都与另一种小鼠物种：小家鼠交叉杂交。在这个物种中，每个片段似乎都位于X染色体上，表明一些X染色体重复序列是部分保守的。此外，一个片段与人类DNA交叉杂交。
