Mileham P, Brown S D
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College of Science, Technology and Medicine, London W2 1PG, UK.
Mamm Genome. 1996 Apr;7(4):253-61. doi: 10.1007/s003359900077.
We have initiated work towards the construction of YAC clone contigs across a repeat sequence island region on the mouse X Chromosome (Chr). The repeat sequence island region-the 141 island-located at band A3 contains 50 copies of a localized long complex repeat unit (LCRU). We have isolated 87 YAC clones from the 141 island and have used a dual faceted approach towards the construction of contigs across the repeat sequence island. First, we have identified YAC clones originating from the same region of the island by the identification of commonly held LCRU restriction site variants. Second, we have constructed rare cutter restriction maps of each YAC clone. Taken together, we have been able to assemble one large contig of 2.8 Mb and a number of smaller contigs. In total, contigs covering 5Mb of the island region have been identified. The island region would appear to represent a major component of the A3 Giemsa dark band on the mouse X Chr.
我们已着手构建跨越小鼠X染色体（Chr）上一个重复序列岛区域的酵母人工染色体（YAC）克隆重叠群。该重复序列岛区域——位于A3带的141号岛——包含50个定位的长复合重复单元（LCRU）拷贝。我们从141号岛分离出了87个YAC克隆，并采用双方面的方法构建跨越该重复序列岛的重叠群。首先，我们通过识别共同拥有的LCRU限制性酶切位点变体，鉴定出来自该岛同一区域的YAC克隆。其次，我们构建了每个YAC克隆的稀有切割限制性图谱。综合起来，我们已能够组装出一个2.8 Mb的大重叠群和一些较小的重叠群。总共已鉴定出覆盖该岛区域5 Mb的重叠群。该岛区域似乎是小鼠X Chr上A3吉姆萨暗带的主要组成部分。
Zotero 插件