Lebedev Aleksey V, Kazennova E V, Zverev S Ya, Nistratova Yu I, Laga V Yu, Tumanov A S, Glushchenko N V, Yarygina E I, Bobkova M R
Vopr Virusol. 2016;61(5):222-9.
In the present work, a total of 132 HIV-1 env gene C2-V3-C3 sequences belonging to the IDU-A genetic variant were analyzed. The variants were obtained from the viruses circulating among IDUs and heterosexuals in the Perm region at different periods. It was shown that the rate of the divergence of the IDU-A HIV-1 viruses from a common ancestor increased 4.3 times (p < 0.001) in 2011 as compared with the onset of the epidemics. The rate of the HIV-1 evolution was different in the two risk groups of the infection. The mean genetic distance of HIV-1 variants circulating among heterosexuals was 1.3 times longer (p = 0.008) than that among IDUs. The accumulation rate of the nucleotide (including nonsynonymous) substitutions in the C2-V3-C3 HIV-1 env gene region among individuals infected by heterosexual contacts was 1.7 times higher than that among IDUs. The differences in the positions of the codons subjected to positive selection were demonstrated depending on the infection risk group tested.
在本研究中，共分析了132个属于IDU-A基因变体的HIV-1 env基因C2-V3-C3序列。这些变体来自不同时期在彼尔姆地区吸毒者和异性恋者中传播的病毒。结果表明，与疫情开始时相比，2011年IDU-A HIV-1病毒与其共同祖先的分歧率增加了4.3倍（p<0.001）。在两个感染风险组中，HIV-1的进化速度不同。异性恋者中传播的HIV-1变体的平均遗传距离比吸毒者中的长1.3倍（p = 0.008）。异性接触感染个体中，HIV-1 env基因C2-V3-C3区域核苷酸（包括非同义）替换的积累率比吸毒者高1.7倍。根据所测试的感染风险组，在接受正选择的密码子位置上存在差异。
