Dedkov Vladimir G, Deviatkin A A, Poleschuk E M, Safonova M V, Markelov M L, Shipulin G A
Vopr Virusol. 2016;61(5):235-40.
To improve the diagnosis, surveillance, and control for the rabies virus, a kit for hybridization-triggered fluorescence detection of rabies virus DNA by the RT-PCR technique was developed and evaluated. The analytical sensitivity of the kit was 4*10 GE per ml. High specificity of the kit was shown using representative sampling of viral, bacterial, and human nucleic acids.
为改善狂犬病病毒的诊断、监测和控制,开发并评估了一种通过逆转录聚合酶链反应(RT-PCR)技术进行狂犬病病毒DNA杂交触发荧光检测的试剂盒。该试剂盒的分析灵敏度为每毫升4×10个荧光单位(GE)。使用病毒、细菌和人类核酸的代表性样本显示该试剂盒具有高特异性。