Widder Edith A, Latz Michael I, Case James F
Biol Bull. 1983 Dec;165(3):791-810. doi: 10.2307/1541479.
The emission spectra of 70 bioluminescent marine species were measured with a computer controlled optical multichannel analyzer (OMA). A 350 nm spectral window is simultaneously measured using a linear array of 700 silicon photodiodes, coupled by fiber optics to a microchannel plate image intensifier on which a polychromator generated spectrum is focused. Collection optics include a quartz fiber optic bundle which allows spectra to be measured from single photophores. Since corrections are not required for temporal variations in emissions, it was possible to acquire spectra of transient luminescent events that would be difficult or impossible to record with conventional techniques. Use of this system at sea on freshly trawled material and in the laboratory has permitted acquisition of a large collection of bioluminescence spectra of precision rarely obtained previously with such material. Among unusual spectral features revealed were organisms capable of emitting more than one color, including: Umbellula magniflora and Stachyptilum superbum (Pennatulacea), Parazoanthus lucificum (Zoantharia), and Cleidopus gloria-maris (Pisces). Evidence is presented that the narrow bandwidth of the emission spectrum for Argyropelecus affinis (Pisces) is due to filters in the photophores.
使用计算机控制的光学多通道分析仪（OMA）测量了70种海洋生物发光物种的发射光谱。利用由700个硅光电二极管组成的线性阵列同时测量350纳米的光谱窗口，该阵列通过光纤耦合到一个微通道板图像增强器上，多色仪产生的光谱聚焦在该增强器上。收集光学器件包括一个石英光纤束，它允许从单个发光体测量光谱。由于不需要对发射的时间变化进行校正，因此有可能获取瞬态发光事件的光谱，而用传统技术记录这些事件将是困难的或不可能的。在海上对刚拖网捕捞的材料以及在实验室中使用该系统，使得能够获得大量生物发光光谱，这些光谱的精度是以前用此类材料很少能达到的。所揭示的不寻常光谱特征包括能够发出不止一种颜色的生物，其中有：大花伞形水母和华丽穗水母（海鳃目）、发光拟海葵（珊瑚虫纲）以及光辉灯笼鱼（硬骨鱼纲）。有证据表明，黑等鳍叉尾带鱼（硬骨鱼纲）发射光谱的窄带宽是由于发光体中的滤光器造成的。
