Guangzhou Institute for Drug Control, Guangzhou 510160, China; State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau, China.
Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China.
J Pharm Biomed Anal. 2018 Mar 20;151:126-132. doi: 10.1016/j.jpba.2017.12.051. Epub 2017 Dec 26.
Ultra-high-performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UHPLC-Q/TOF-MS) in positive ion mode was used to profile and identify the major constituents in Euphorbia fischeriana Steud. (E. fischeriana). An Agilent ZORBAX SB-C column was used to separate the crude extract of E. fischeriana, and the mobile phases were acetonitrile and 0.1% aqueous formic acid (v/v). A total of 40 peaks were detected, and 37 components, including phloroglucinol derivatives, diterpenoids and nitrogenous compounds, were identified based on their accurate masses and fragmentation characteristics. The chemical structures of 7 of the detected compounds were determined by comparing standard compounds to the compounds that were isolated from E. fischeriana. Reasonable fragmentation mechanisms and key fragment ions of the four jolkinolide components were determined to aid in the identification of the other diterpenoids in E. fischeriana. In addition, the compound corresponding to peak 18, namely, jolkinomethylide, was determined to be new, and its chemical structure was determined and elucidated based on MS and NMR spectroscopic data. The present study provided valuable information on the new components in E. fischeriana and established a practical method for evaluating the quality of E. fischeriana.
采用正离子模式的超高效液相色谱-四极杆/飞行时间质谱联用技术（UHPLC-Q/TOF-MS）对大戟属植物Euphorbia fischeriana Steud.（E. fischeriana）的主要成分进行了分析和鉴定。采用安捷伦 ZORBAX SB-C 柱对大戟属植物的粗提取物进行分离，流动相为乙腈和 0.1%甲酸水溶液（v/v）。共检测到 40 个峰，根据精确质量和裂解特征鉴定出 37 种成分，包括苯丙三醇衍生物、二萜类和含氮化合物。通过将检测到的化合物与从大戟属植物中分离出的化合物进行比较，确定了其中 7 种化合物的化学结构。确定了 4 种 Jolkinolide 成分的合理裂解机制和关键碎片离子，以帮助鉴定大戟属植物中的其他二萜类化合物。此外，峰 18 对应的化合物，即 Jolkinomethylide，被确定为新化合物，并根据 MS 和 NMR 光谱数据确定并阐明了其化学结构。本研究为大戟属植物的新成分提供了有价值的信息，并建立了一种评估大戟属植物质量的实用方法。
