Research Institute of Medicine and Pharmacy, Qiqihar Medical University, Bukui Road 333, Qiqihar 161006, China.
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang 110016, China.
Molecules. 2022 Jul 3;27(13):4282. doi: 10.3390/molecules27134282.
Ent-abietane diterpenoids are the main active constituents of . In the continuing search for new anti-breast cancer drugs, 11 ent-abietane diterpenoids (-) were isolated from . The structures of these compounds were clearly elucidated on the basis of 1D and 2D NMR spectra as well as HRESIMS data. Among them, compound was a novel compound, compound was isolated from genus for the first time, compound was firstly discovered from . . These compounds exhibited varying degrees of growth inhibition against the MCF-10A, MCF-7, ZR-75-1 and MDA-MB-231 cell lines in vitro. The experimental data obtained permit us to identify the roles of the epoxy group, hydroxyl group and acetoxyl group on their cytotoxic activities. Extraction is an important means for the isolation, identification, and application of valuable compounds from natural plants. To maximize yields of ent-abietane diterpenoids of , 17-hydroxyjolkinolide B, jolkinolide B, 17-hydroxyjolkinolide A and jolkinolide A were selected as quality controls to optimize the salting-out-assisted liquid-liquid extraction (SALLE) by response surface methodology (RSM). The optimized conditions for SALLE were 0.47 g sodium dihydrogen phosphate, 5.5 mL acetonitrile and 4.5 mL water at pH 7.5. The experimental values of 17-hydroxyjolkinolide B, jolkinolide B, 17-hydroxyjolkinolide A and jolkinolide A (2.134, 0.529, 0.396, and 0.148 mg/g, respectively) were in agreement with the predicted values, thus demonstrating the appropriateness of the model.
松香烷二萜类化合物是. 的主要活性成分。在继续寻找新的抗乳腺癌药物的过程中，从. 中分离得到了 11 种松香烷二萜类化合物（-）。这些化合物的结构是根据 1D 和 2D NMR 光谱以及 HRESIMS 数据明确阐明的。其中，化合物 是一种新化合物，化合物 是首次从. 属中分离得到的，化合物 是首次从. 中发现的。这些化合物在体外对 MCF-10A、MCF-7、ZR-75-1 和 MDA-MB-231 细胞系表现出不同程度的生长抑制作用。实验数据使我们能够确定环氧基、羟基和乙酰氧基对其细胞毒性活性的作用。提取是从天然植物中分离、鉴定和应用有价值化合物的重要手段。为了最大限度地提高. 中松香烷二萜的产量，选择 17-羟基乔卡林内酯 B、乔卡林内酯 B、17-羟基乔卡林内酯 A 和乔卡林内酯 A 作为质量控制物，通过响应面法（RSM）优化盐析辅助液-液萃取（SALLE）。SALLE 的优化条件为 0.47 g 磷酸二氢钠、5.5 mL 乙腈和 4.5 mL pH7.5 的水。17-羟基乔卡林内酯 B、乔卡林内酯 B、17-羟基乔卡林内酯 A 和乔卡林内酯 A（分别为 2.134、0.529、0.396 和 0.148 mg/g）的实验值与预测值一致，因此证明了模型的适宜性。
