Department of Membrane Transport, Institute of Physiology of the Czech Academy of Sciences, 14220 Prague 4, Czech Republic.
Food Biotechnology Department, Systems Biology in Yeast of Biotechnological Interest, Instituto de Agroquímica y Tecnología de los Alimentos, CSIC, 46980 Paterna, Valencia, Spain.
Int J Food Microbiol. 2018 Mar 2;268:27-34. doi: 10.1016/j.ijfoodmicro.2018.01.002. Epub 2018 Jan 5.
S. kudriavzevii has potential for fermentations and other biotechnological applications, but is sensitive to many types of stress. We tried to increase its tolerance and performance via the expression of various transporters from different yeast species. Whereas the overexpression of Z. rouxii fructose uptake systems (ZrFfz1 and ZrFsy1) or a glycerol importer (ZrStl1) did not improve the ability of S. kudriavzevii to consume fructose and survive osmotic stress, the expression of alkali-metal-cation exporters (ScEna1, ScNha1, YlNha2) improved S. kudriavzevii salt tolerance, and that of ScNha1 also the fermentation performance. The level of improvement depended on the type and activity of the transporter suggesting that the natural sensitivity of S. kudriavzevii cells to salts is based on a non-optimal functioning of its own transporters.
S. kudriavzevii 具有发酵和其他生物技术应用的潜力，但对许多类型的压力敏感。我们试图通过表达来自不同酵母物种的各种转运蛋白来提高其耐受性和性能。然而，过量表达 Z. rouxii 果糖摄取系统（ZrFfz1 和 ZrFsy1）或甘油渗透酶（ZrStl1）并没有提高 S. kudriavzevii 消耗果糖和耐受渗透压的能力，而碱金属阳离子外排蛋白（ScEna1、ScNha1、YlNha2）的表达提高了 S. kudriavzevii 的耐盐性，ScNha1 的表达也提高了发酵性能。改进的程度取决于转运蛋白的类型和活性，这表明 S. kudriavzevii 细胞对盐的天然敏感性是基于其自身转运蛋白的功能失调。
