Deng Tian-Ci, Yang Ji-Yun, Sun Mei-Ling, Zhang Yun-Zhao, Pan Yun-Ting, Huang Lin
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
J Fungi (Basel). 2023 May 13;9(5):566. doi: 10.3390/jof9050566.
, a significant fungal pathogen of crops and trees, causes large economic losses worldwide. However, its pathogenic mechanism remains totally unclear. In this study, four Ena ATPases (-type adenosine triphosphatases), homology of yeast Ena proteins, were identified in . Gene deletion mutants of Δ, Δ, Δ, and Δ were obtained through the method of gene replacement. First, a subcellular localization pattern indicated that CgEna1 and CgEna4 were localized in the plasma membrane, while the CgEna2 and CgEna3 were distributed in the endoparasitic reticulum. Next, it was found that CgEna1 and CgEna4 were required for sodium accumulation in . CgEna3 was required for extracellular ion stress of sodium and potassium. CgEna1 and CgEna3 were involved in conidial germination, appressorium formation, invasive hyphal development, and full virulence. The mutant of Δ was more sensitive to the conditions of high concentrations of ion and the alkaline. Together, these results indicated that CgEna ATPase proteins have distinct roles in sodium accumulation, stress resistance, and full virulence in .
是一种重要的农作物和树木真菌病原体，在全球范围内造成巨大经济损失。然而，其致病机制仍完全不清楚。在本研究中，在中鉴定出四个与酵母Ena蛋白同源的Ena ATP酶（-型三磷酸腺苷酶）。通过基因替换方法获得了Δ、Δ、Δ和Δ的基因缺失突变体。首先，亚细胞定位模式表明CgEna1和CgEna4定位于质膜，而CgEna2和CgEna3分布在内质网。接下来，发现CgEna1和CgEna4是中钠积累所必需的。CgEna3是钠和钾细胞外离子应激所必需的。CgEna1和CgEna3参与分生孢子萌发、附着胞形成、侵入菌丝发育和完全毒力。Δ突变体对高浓度离子和碱性条件更敏感。总之，这些结果表明CgEna ATP酶蛋白在的钠积累、抗逆性和完全毒力中具有不同作用。
