Mehari Teame Gereziher, Xu Yanchao, Umer Muhammad Jawad, Shiraku Margaret Linyerera, Hou Yuqing, Wang Yuhong, Yu Shuxun, Zhang Xianlong, Wang Kunbo, Cai Xiaoyan, Zhou Zhongli, Liu Fang
State Key Laboratory of Cotton Biology, Cotton Institute of the Chinese Academy of Agricultural Sciences, Anyang, China.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, China.
Front Plant Sci. 2021 Oct 21;12:746771. doi: 10.3389/fpls.2021.746771. eCollection 2021.
Cotton is one of the most important fiber crops globally. Despite this, various abiotic stresses, including drought, cause yield losses. We used transcriptome profiles to investigate the co-expression patterns of gene networks associated with drought stress tolerance. We identified three gene modules containing 3,567 genes highly associated with drought stress tolerance. Within these modules, we identified 13 hub genes based on intramodular significance, for further validation. The yellow module has five hub genes (, and ), the brown module contains three hub genes belonging to the aldehyde dehydrogenase (ALDH) gene family (, and ), and the pink module has five hub genes (, and ). Based on RT-qPCR results, the gene has the highest expression under drought stress in different plant tissues and it might be the true candidate gene linked to drought stress tolerance in cotton. Silencing of in cotton leaves conferred significant sensitivity in response to drought stress treatments. Overexpression of in Arabidopsis also confirms its role in drought stress tolerance. L-valine, Glutaric acid, L-proline, L-Glutamic acid, and L-Tryptophan were found to be the most significant metabolites playing roles in drought stress tolerance. These findings add significantly to existing knowledge of drought stress tolerance mechanisms in cotton.
棉花是全球最重要的纤维作物之一。尽管如此，包括干旱在内的各种非生物胁迫仍会导致产量损失。我们利用转录组图谱来研究与耐旱性相关的基因网络的共表达模式。我们鉴定出三个包含3567个与耐旱性高度相关基因的基因模块。在这些模块中，我们基于模块内显著性鉴定出13个中心基因，以供进一步验证。黄色模块有5个中心基因（……），棕色模块包含3个属于醛脱氢酶（ALDH）基因家族的中心基因（……），粉色模块有5个中心基因（……）。基于逆转录定量聚合酶链反应（RT-qPCR）结果，……基因在不同植物组织的干旱胁迫下表达量最高，它可能是棉花中与耐旱性相关的真正候选基因。在棉花叶片中沉默……会使其在干旱胁迫处理下表现出显著的敏感性。在拟南芥中过表达……也证实了其在耐旱性中的作用。发现L-缬氨酸、戊二酸、L-脯氨酸、L-谷氨酸和L-色氨酸是在耐旱性中起作用的最显著代谢物。这些发现极大地丰富了棉花耐旱胁迫耐受机制的现有知识。
