Li Xiu-Mei, Liu Jing, Pan Fang-Fang, Shi Dong-Dong, Wen Zhi-Guo, Yang Pei-Long
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
PLoS One. 2018 Jan 11;13(1):e0191062. doi: 10.1371/journal.pone.0191062. eCollection 2018.
Up till now, studies have not been conducted on how the combination of Quercetin (Q), Aconitine (A) and apoptosis induction affects human cervical carcinoma HeLa cells. The result of our findings shows that the combination of Q and A (QA) is capable of synergistically inhibiting the proliferation of HeLa cells in a number of concentrations. QA synergistically inhibits the proliferation of MDR1 gene in the HeLa cells. It is concluded based on our result that QA induces apoptosis and ER stress just as QA-induced ER stress pathway may mediate apoptosis by upregulating mRNA expression levels of eIF2α, ATF4, IRE1, XBP1, ATF6, PERK and CHOP in the HeLa cells. The up-regulating of mRNA expression level of GRP78 and activation of UPR are a molecular basis of QA-induced ER stress.
迄今为止，尚未开展关于槲皮素（Q）、乌头碱（A）联合应用及诱导凋亡对人宫颈癌HeLa细胞影响的研究。我们的研究结果表明，Q与A联合（QA）在多种浓度下均能协同抑制HeLa细胞增殖。QA协同抑制HeLa细胞中MDR1基因的增殖。根据我们的结果得出结论，QA诱导凋亡和内质网应激，正如QA诱导的内质网应激途径可能通过上调HeLa细胞中eIF2α、ATF4、IRE1、XBP1、ATF6、PERK和CHOP的mRNA表达水平来介导凋亡一样。GRP78 mRNA表达水平的上调和未折叠蛋白反应的激活是QA诱导内质网应激的分子基础。
