Institute of Pharmaceutical Research and DevelopmentCollege of Pharmacy, Wonkwang University, Iksan, Jeonbuk, South Korea.
Hanpoong Pharm. Co. LtdJeonju-si, Jeonbuk, South Korea.
Reproduction. 2018 Feb;155(2):105-115. doi: 10.1530/REP-17-0530.
HPC 03 is herbal formula that consists of extracts from , Makino and Presl. The present study evaluated the estrogenic potential of HPC 03 by using and models. The regulatory mechanisms of HPC 03 in estrogen-dependent MCF-7 cells were assessed. HPC 03 induced the proliferation of estrogen receptor-positive MCF-7 cells, and the proliferation was blocked by the addition of the estrogen antagonist tamoxifen. The estrogen receptor luciferase activities were significantly increased by HPC 03 treatment, which also increased the mRNA expression of the estrogen-responsive genes , and Also, we evaluated the ameliorative effects of HPC 03 on menopausal symptoms in ovariectomized rats. HPC 03 treatment in OVX rats significantly affected the uterine weight, increased the expression of estrogen-responsive genes and in uterus, increased bone mineral density loss in the femur and inhibited body weight increase. Serum E2, collagen type 1 and osteocalcin were significantly increased, while serum LH, FSH and ALP were decreased compared with OVX rats. HPC 03 may be a promising candidate for the treatment of menopause, but further research is necessary to determine whether the observed effects also occur in humans.
HPC 03 是一种草药配方，由 、 和 提取物组成。本研究通过 和 模型评估了 HPC 03 的雌激素潜力。评估了 HPC 03 在雌激素依赖性 MCF-7 细胞中的调节机制。HPC 03 诱导雌激素受体阳性 MCF-7 细胞的增殖，并且这种增殖可被雌激素拮抗剂他莫昔芬阻断。HPC 03 处理显著增加了雌激素受体荧光素酶活性，同时也增加了雌激素反应基因 、 和 的 mRNA 表达。此外，我们评估了 HPC 03 对去卵巢大鼠更年期症状的改善作用。HPC 03 处理在 OVX 大鼠中显著影响子宫重量，增加了子宫中雌激素反应基因 和 的表达，抑制了股骨骨矿物质密度的丢失，并抑制了体重的增加。与 OVX 大鼠相比，血清 E2、胶原 1 型和骨钙素显著增加，而血清 LH、FSH 和 ALP 降低。HPC 03 可能是治疗更年期的有前途的候选药物，但需要进一步研究确定在人类中是否也观察到这些作用。
