School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, P. R. China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
Adv Mater. 2018 Feb;30(8). doi: 10.1002/adma.201705418. Epub 2018 Jan 12.
Remote control and noninvasive manipulation of cellular bioprocess has received intensive attention as a powerful technology to control cell functions. Here, a strategy is developed to remotely control intracellular gene expression with high spatial and temporal resolutions by using photothermal-responsive conjugated polymer nanoparticles (CPNs) as the transducer under near-infrared light irradiation. After being modified with positive charged peptide, the CPNs with superior photothermal conversion capacity could effectively coat on the surface of living cells and generate localized heat to trigger target gene expression. The heat-inducible heat shock protein-70 promoter starts transcription of downstream EGFP gene in response to heat shock, thus producing green fluorescent protein in the living cells. The combination of heat-inducible gene promoter and photothermal-responsive CPNs provides a method for the development of thermogenetics.
远程控制和非侵入式的细胞生物过程操控作为一种控制细胞功能的强大技术受到了广泛关注。在这里，我们开发了一种策略，通过使用光热响应共轭聚合物纳米粒子（CPNs）作为近红外光照射下的换能器，以高时空分辨率远程控制细胞内基因表达。经过正电荷肽修饰后，具有优异光热转换能力的 CPNs 可以有效地包覆在活细胞表面，并产生局部热量以触发靶基因表达。热诱导的热休克蛋白-70 启动子在受到热冲击时会启动下游 EGFP 基因的转录，从而在活细胞中产生绿色荧光蛋白。热诱导基因启动子和光热响应 CPNs 的结合为热遗传学的发展提供了一种方法。
