盐酸利多卡因诱导 SH-SY5Y 细胞 Cav3.1 表达上调。
Up-regulation of Cav3.1 expression in SH-SY5Y cells induced by lidocaine hydrochloride.
机构信息
a Department of Pain Management , The First Affiliated Hospital of Jinan University , Guangzhou , Guangdong Province , China.
b Department of Anesthesiology , Foshan Hospital of Traditional Chinese Medicine , Foshan , Guangdong Province , China.
出版信息
Artif Cells Nanomed Biotechnol. 2018;46(sup1):372-379. doi: 10.1080/21691401.2018.1425697. Epub 2018 Jan 12.
BACKGROUND
Neurotoxicity induced by the local anaesthetics has aroused concern. A previous study has shown that an overload of intracellular calcium was involved in the neurotoxic effect. Cav3.1 is one of the low-voltage-activated (LVA) calcium channels which play a key point to regulate the intracellular calcium ion level. This study aimed to investigate the changes of the Cav3.1 expression in the SH-SY5Y cells treated with lidocaine hydrochloride.
METHODS
The SH-SY5Y cells were treated with different concentrations of lidocaine hydrochloride(1 mM, 5 mM and 10 mM, namely L1 group, L5 group and L10 group) and different exposure times (1 h,12 h and 24 h), respectively. Cell viability, Cav3.1 protein and mRNA expression were detected.
RESULTS
The results showed that cell viability decreased and Cav3.1 mRNA and protein expression increased with the concentration (from 1 mM to 10 mM) of the lidocaine hydrochloride and exposure time (from 1 h to 24 h) to the SH-SY5Y cell line increased.
CONCLUSION
Those data showed that lidocaine hydrochloride induced SH-SY5Y cell toxicity and up-regulated Cav3.1mRNA and protein expression.
背景
局部麻醉剂引起的神经毒性引起了关注。先前的研究表明，细胞内钙离子过载参与了神经毒性作用。Cav3.1 是一种低电压激活 (LVA) 钙通道，对调节细胞内钙离子水平起着关键作用。本研究旨在探讨盐酸利多卡因处理的 SH-SY5Y 细胞中 Cav3.1 表达的变化。
方法
用不同浓度的盐酸利多卡因（1mM、5mM 和 10mM，分别为 L1 组、L5 组和 L10 组）和不同暴露时间（1h、12h 和 24h）处理 SH-SY5Y 细胞。检测细胞活力、Cav3.1 蛋白和 mRNA 表达。
结果
结果表明，随着盐酸利多卡因浓度（从 1mM 到 10mM）和暴露时间（从 1h 到 24h）的增加，细胞活力降低，Cav3.1mRNA 和蛋白表达增加。
结论
这些数据表明，盐酸利多卡因诱导 SH-SY5Y 细胞毒性，并上调 Cav3.1mRNA 和蛋白表达。
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