Tran Dinh Minh, Sugimoto Hayuki, Nguyen Dzung Anh, Watanabe Takeshi, Suzuki Kazushi
a Graduate School of Science and Technology , Niigata University , Niigata , Japan.
b Institute of Biotechnology and Environment , Tay Nguyen University , Buon Ma Thuot City , Vietnam.
Biosci Biotechnol Biochem. 2018 Feb;82(2):343-355. doi: 10.1080/09168451.2017.1422969. Epub 2018 Jan 12.
To develop a novel type of biocontrol agent, we focus on bacteria that are characterized by both chitinase activity and biofilm development. Chitinolytic bacteria were isolated from sediments and chitin flakes immersed in the water of a sand dune lake, Sakata, in Niigata, Japan. Thirty-one isolates from more than 5100 isolated strains were examined chitinase activity and biofilm formation. Phylogenetic analysis of these isolates based on the 16S rRNA gene sequences revealed that most isolates belonged to the family Aeromonadaceae, followed by Paenibacillaceae, Enterobacteriaceae, and Neisseriaceae. The specific activity of chitinase of four selected strains was higher than that of a reference strain. The molecular size of one chitinase produced by Andreprevotia was greater than that of typical bacterial chitinases. The dialyzed culture supernatant containing chitinases of the four strains suppressed hyphal growth of Trichoderma reesei. These results indicate that these four strains are good candidates for biocontrol agents.
为开发一种新型生物防治剂，我们聚焦于具有几丁质酶活性和生物膜形成特性的细菌。从日本新潟县坂田沙丘湖水中浸泡的沉积物和几丁质薄片中分离出了几丁质分解细菌。在5100多个分离菌株中，对31个分离株进行了几丁质酶活性和生物膜形成检测。基于16S rRNA基因序列对这些分离株进行系统发育分析，结果表明大多数分离株属于气单胞菌科，其次是芽孢杆菌科、肠杆菌科和奈瑟菌科。四个选定菌株的几丁质酶比活性高于参考菌株。Andreprevotia产生的一种几丁质酶的分子大小大于典型细菌几丁质酶。含有这四个菌株几丁质酶的透析培养上清液抑制了里氏木霉的菌丝生长。这些结果表明这四个菌株是生物防治剂的良好候选菌株。
