Tran Dinh Minh, Huynh To Uyen, Do Tu Oanh
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Viet Nam.
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, Dak Lak 630000, Viet Nam.
J Genet Eng Biotechnol. 2024 Sep;22(3):100402. doi: 10.1016/j.jgeb.2024.100402. Epub 2024 Jul 22.
Bacillus velezensis RB.IBE29 harbors two chitinases belonging to the glycoside hydrolase family 18 and exhibiting a novel domain structure. The roles of these chitinases in crop production have been reported; nevertheless, their contribution to controlling human pathogens is unknown. In this initial work, the chitinases A (BvChiA) and B (BvChiB) of strain RB.IBE29 were produced in recombinant Escherichia coli BL21-CodonPlus (DE3)-RIPL cells and subsequently purified using HisTrap FF column. The purified BvChiA and BvChiB exhibited the highest chitinase and binding activities against colloidal chitin. Combining both chitinases for the hydrolysis of powdered chitin increased the reducing sugar content by 88.7 %. Moreover, the purified chitinases remarkably suppressed the germination of Candida albicans VTCC 20568 (=JCM 2070) cells. These results indicated that the novel domain-structure-containing chitinases of strain RB.IBE29 have great potential and can be further developed as a novel therapeutic agent against human pathogenic C. albicans.
贝莱斯芽孢杆菌RB.IBE29含有两种属于糖苷水解酶家族18且具有新型结构域结构的几丁质酶。这些几丁质酶在作物生产中的作用已有报道；然而，它们对控制人类病原体的贡献尚不清楚。在这项初步研究中，菌株RB.IBE29的几丁质酶A（BvChiA）和B（BvChiB）在重组大肠杆菌BL21-CodonPlus（DE3）-RIPL细胞中产生，随后使用HisTrap FF柱进行纯化。纯化后的BvChiA和BvChiB对胶体几丁质表现出最高的几丁质酶活性和结合活性。将两种几丁质酶结合用于水解粉状几丁质，使还原糖含量提高了88.7%。此外，纯化后的几丁质酶显著抑制了白色念珠菌VTCC 20568（=JCM 2070）细胞的萌发。这些结果表明，菌株RB.IBE29中含有新型结构域的几丁质酶具有巨大潜力，可进一步开发成为一种新型抗人类致病性白色念珠菌的治疗剂。
