λ位点特异性重组中方向性的控制
Control of directionality in lambda site specific recombination.
作者信息
Bushman W, Thompson J F, Vargas L, Landy A
出版信息
Science. 1985 Nov 22;230(4728):906-11. doi: 10.1126/science.2932798.
Abstract
The simple relation between the substrates and products of site-specific recombination raises questions about the control of directionality often observed in this class of DNA transactions. For bacteriophage lambda, viral integration and excision proceed by discrete pathways, and DNA substrates with the intrinsic property of recombining in only one direction can be constructed. These pathways display an asymmetric reliance on a complex array of protein binding sites, and they respond differently to changes in the concentrations of the relevant proteins. The Escherichia coli protein integration host factor (IHF) differentially affects integrative and excisive recombination, thereby influencing directionality. A four- to eightfold increase in intracellular IHF coincides with the transition from exponential to stationary phase; this provides a mechanism for growth phase-dependent regulation of recombination that makes the cellular physiology an intrinsic part of the recombination reaction.
摘要
位点特异性重组的底物与产物之间的简单关系引发了有关这类DNA交易中常观察到的方向性控制的问题。对于噬菌体λ而言，病毒整合和切除通过离散途径进行，并且可以构建仅在一个方向上进行重组的具有内在特性的DNA底物。这些途径对一系列复杂的蛋白质结合位点表现出不对称的依赖性，并且它们对相关蛋白质浓度的变化反应不同。大肠杆菌蛋白整合宿主因子（IHF）对整合性和切除性重组有不同影响，从而影响方向性。细胞内IHF增加四至八倍与从指数生长期到稳定期的转变同时发生；这为重组的生长阶段依赖性调节提供了一种机制，使细胞生理学成为重组反应的内在组成部分。
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