Li Ying, Di Jianglei, Ma Chaojie, Zhang Jiwei, Zhong Jinzhan, Wang Kaiqiang, Xi Teli, Zhao Jianlin
Opt Express. 2018 Jan 8;26(1):586-593. doi: 10.1364/OE.26.000586.
We demonstrate a simple method for quantitative phase imaging of tiny transparent objects such as living cells based on the transport of intensity equation. The experiments are performed using an inverted bright field microscope upgraded with a flipping imaging module, which enables to simultaneously create two laterally separated images with unequal defocus distances. This add-on module does not include any lenses or gratings and is cost-effective and easy-to-alignment. The validity of this method is confirmed by the measurement of microlens array and human osteoblastic cells in culture, indicating its potential in the applications of dynamically measuring living cells and other transparent specimens in a quantitative, non-invasive and label-free manner.
我们展示了一种基于强度传输方程对微小透明物体（如活细胞）进行定量相位成像的简单方法。实验是使用配备翻转成像模块升级后的倒置明场显微镜进行的，该模块能够同时创建两个横向分离且散焦距离不等的图像。这个附加模块不包括任何透镜或光栅，具有成本效益且易于校准。通过对微透镜阵列和培养中的人成骨细胞的测量，证实了该方法的有效性，表明其在以定量、非侵入性和无标记方式动态测量活细胞及其他透明标本方面的潜力。
