Chen Chao, Lu Yu-Nan, Huang Huachuan, Yan Keding, Jiang Zhilong, He Xiaoliang, Kong Yan, Liu Cheng, Liu Fei, Xue Liang, Wang Shouyu
Computational Optics Laboratory, School of Sciences, Jiangnan University, Wuxi, Jiangsu 214122, China.
Single Molecule Nanometry Laboratory (Sinmolab), Nanjing Agricultural University, Nanjing 210095, China.
Biomed Opt Express. 2021 Jul 26;12(8):5261-5271. doi: 10.1364/BOE.430115. eCollection 2021 Aug 1.
We design a novel phase real-time microscope camera (PhaseRMiC) for live cell phase imaging. PhaseRMiC has a simple and cost-effective configuration only consisting of a beam splitter and a board-level camera with two CMOS imaging chips. Moreover, integrated with 3-D printed structures, PhaseRMiC has a compact size of 136×91×60 mm, comparable to many commercial microscope cameras, and can be directly connected to the microscope side port. Additionally, PhaseRMiC can be well adopted in real-time phase imaging proved with satisfied accuracy, good stability and large field of view. Considering its compact and cost-effective device design as well as real-time phase imaging capability, PhaseRMiC is a preferred solution for live cell imaging.
我们设计了一种用于活细胞相位成像的新型相位实时显微镜相机（PhaseRMiC）。PhaseRMiC具有简单且经济高效的配置，仅由一个分束器和一个带有两个CMOS成像芯片的板级相机组成。此外，与3D打印结构集成后，PhaseRMiC的紧凑尺寸为136×91×60毫米，与许多商用显微镜相机相当，并且可以直接连接到显微镜侧端口。此外，PhaseRMiC在实时相位成像中表现出色，具有令人满意的精度、良好的稳定性和大视野。考虑到其紧凑且经济高效的设备设计以及实时相位成像能力，PhaseRMiC是活细胞成像的首选解决方案。
