单细胞基因组学：未来免疫学发现的踏脚石。

Single-Cell Genomics: A Stepping Stone for Future Immunology Discoveries.

机构信息

Department of Immunology, Weizmann Institute, Rehovot 76100, Israel.

出版信息

Cell. 2018 Jan 11;172(1-2):14-21. doi: 10.1016/j.cell.2017.11.011.

DOI:10.1016/j.cell.2017.11.011

Abstract

The immunology field has invested great efforts and ingenuity to characterize the various immune cell types and elucidate their functions. However, accumulating evidence indicates that current technologies and classification schemes are limited in their ability to account for the functional heterogeneity of immune processes. Single-cell genomics hold the potential to revolutionize the way we characterize complex immune cell assemblies and study their spatial organization, dynamics, clonal distribution, pathways, function, and crosstalks. In this Perspective, we consider recent and forthcoming technological and analytical advances in single-cell genomics and the potential impact of those advances on the future of immunology research and immunotherapy.

摘要

免疫学领域投入了大量的精力和创造力来描述各种免疫细胞类型并阐明它们的功能。然而，越来越多的证据表明，当前的技术和分类方案在解释免疫过程的功能异质性方面能力有限。单细胞基因组学有可能彻底改变我们描述复杂免疫细胞组合的方式，并研究它们的空间组织、动态、克隆分布、途径、功能和串扰。在本观点中，我们考虑了单细胞基因组学中最近和即将出现的技术和分析进展，以及这些进展对免疫学研究和免疫治疗未来的潜在影响。