Institute of Nano-, and Biotechnologies (INB), Aachen University of Applied Sciences, Campus Jülich, Heinrich-Mußmann-Str.1, 52428 Jülich, Germany; Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marbacher Weg 6-10, 35032 Marburg, Germany.
Elopak EQS GmbH, Hanns-Martin-Schleyer-Str. 17, 41199 Mönchengladbach, Germany.
Biosens Bioelectron. 2018 May 1;104:87-94. doi: 10.1016/j.bios.2017.12.045. Epub 2017 Dec 27.
In this work, a spore-based biosensor is evaluated to monitor the microbicidal efficacy of sterilization processes applying gaseous hydrogen peroxide (HO). The sensor is based on interdigitated electrode structures (IDEs) that have been fabricated by means of thin-film technologies. Impedimetric measurements are applied to study the effect of sterilization process on spores of Bacillus atrophaeus. This resilient microorganism is commonly used in industry to proof the sterilization efficiency. The sensor measurements are accompanied by conventional microbiological challenge tests, as well as morphological characterizations with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The sensor measurements are correlated with the microbiological test routines. In both methods, namely the sensor-based and microbiological one, a tailing effect has been observed. The results are evaluated and discussed in a three-dimensional calibration plot demonstrating the sensor's suitability to enable a rapid process decision in terms of a successfully performed sterilization.
在这项工作中，评估了一种基于孢子的生物传感器，以监测应用气态过氧化氢 (HO) 的灭菌过程的杀菌效果。该传感器基于叉指电极结构 (IDE)，这些结构是通过薄膜技术制造的。阻抗测量用于研究灭菌过程对萎缩芽孢杆菌孢子的影响。这种有弹性的微生物通常用于工业中以证明灭菌效率。传感器测量伴随着传统的微生物挑战测试，以及扫描电子显微镜 (SEM) 和透射电子显微镜 (TEM) 的形态特征。传感器测量与微生物测试程序相关。在这两种方法中，即基于传感器的方法和微生物学方法，都观察到了拖尾效应。结果在三维校准图中进行了评估和讨论，该图表明传感器适用于根据成功进行的灭菌快速做出过程决策。
