组合量热法气体和孢子基生物传感器阵列，用于无菌灌装机中气态过氧化氢的在线监测和无菌保证。
Combined calorimetric gas- and spore-based biosensor array for online monitoring and sterility assurance of gaseous hydrogen peroxide in aseptic filling machines.
机构信息
Institute of Nano- and Biotechnologies (INB), Aachen University of Applied Sciences, Campus Jülich, 52428, Jülich, Germany; Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marbacher Weg 6-10, 35032 Marburg, Germany.
Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marbacher Weg 6-10, 35032 Marburg, Germany.
出版信息
Biosens Bioelectron. 2019 Oct 15;143:111628. doi: 10.1016/j.bios.2019.111628. Epub 2019 Aug 22.
A combined calorimetric gas- and spore-based biosensor array is presented in this work to monitor and evaluate the sterilization efficacy of gaseous hydrogen peroxide in aseptic filling machines. HO has been successfully measured under industrial conditions. Furthermore, the effect of HO on three different spore strains , namely Bacillus atrophaeus, Bacillus subtilis and Geobacillus stearothermophilus, has been investigated by means of SEM, AFM and impedimetric measurements. In addition, the sterilization efficacy of a spore-based biosensor and the functioning principle are addressed and discussed: the sensor array is convenient to be used in aseptic food industry to guarantee sterile packages.
本工作提出了一种组合量热法气体和孢子生物传感器阵列，用于监测和评估无菌灌装机中气态过氧化氢的灭菌效果。在工业条件下成功测量了 HO。此外，通过 SEM、AFM 和阻抗测量研究了 HO 对三种不同孢子菌株，即萎缩芽孢杆菌、枯草芽孢杆菌和嗜热脂肪芽孢杆菌的影响。此外，还讨论了基于孢子的生物传感器的灭菌效果和工作原理：传感器阵列方便用于无菌食品工业，以保证无菌包装。
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