YongLin Biomedical Engineering Center, National Taiwan University, Taipei City 10617, Taiwan.
Research and Development Department, GenMont Biotech Incorporation, Tainan 741, Taiwan.
J Nutr Biochem. 2018 Apr;54:87-94. doi: 10.1016/j.jnutbio.2017.11.004. Epub 2017 Nov 16.
Obesity is a serious and costly issue to the medical welfare worldwide. Probiotics have been suggested as one of the candidates to resolve the obesity-associated problems, but how they combat obesity is not fully understood. Herein, we investigated the effects of Lactobacillus reuteri 263 (L. reuteri 263) on antiobesity using four groups of Sprague-Dawley rats (n=10/group), namely, C (normal diet with vehicle treatment), HE [high-energy diet (HED) with vehicle treatment], 1X (HED with 2.1×10 CFU/kg/day of L. reuteri 263) and 5X (HED with 1.05×10 CFU/kg/day of L. reuteri 263), for 8 weeks. L. reuteri 263 improved the phenomenon of obesity, serum levels of proinflammatory factors and antioxidant enzymes. More importantly, L. reuteri 263 increased oxygen consumption in white adipose tissue (WAT). The mRNA expressions of thermogenesis genes uncoupling protein-1, uncoupling protein-3, carnitine palmitoyltransferase-1 and cell death-inducing DFFA-like effector-a were up-regulated in WAT of the 5X group. Moreover, L. reuteri 263 might induce browning of WAT due to the higher mRNA levels of browning-related genes peroxisome proliferator-activated receptor-γ, PR domain containing-16, Pparγ coactivator-1α, bone morphogenetic protein-7 and fibroblast growth factor-21 in the 1X and 5X groups compared to the HE group. Finally, L. reuteri 263 altered the expressions of genes involved in glucose and lipid metabolisms in WAT, including increasing the levels of glucose transporter type 4 and carbohydrate-responsive element-binding protein and decreasing the expression of Acetyl-CoA carboxylase-1. The results suggest that L. reuteri 263 may treat obesity through energy metabolism remodeling of WAT in the high-energy-diet-induced obese rats.
肥胖是全球医疗福利的一个严重且代价高昂的问题。益生菌已被提议作为解决肥胖相关问题的候选方案之一，但它们如何对抗肥胖仍未完全了解。在此，我们使用四组 Sprague-Dawley 大鼠（每组 10 只，n=10/group）研究了罗特氏乳杆菌 263（L. reuteri 263）对肥胖的影响，这四组分别为：C（正常饮食，用载体处理）、HE [高能量饮食（HED），用载体处理]、1X（HED 用 2.1×10 CFU/kg/天的 L. reuteri 263 处理）和 5X（HED 用 1.05×10 CFU/kg/天的 L. reuteri 263 处理），持续 8 周。L. reuteri 263 改善了肥胖现象、血清中促炎因子和抗氧化酶的水平。更重要的是，L. reuteri 263 增加了白色脂肪组织（WAT）中的耗氧量。5X 组 WAT 中解偶联蛋白-1、解偶联蛋白-3、肉碱棕榈酰转移酶-1 和细胞死亡诱导 DFFA 样效应因子-a 的生热基因表达上调。此外，由于 1X 和 5X 组的 WAT 中与棕色化相关的基因过氧化物酶体增殖物激活受体-γ、PR 结构域包含蛋白 16、Pparγ 共激活因子 1α、骨形态发生蛋白-7 和成纤维细胞生长因子-21 的 mRNA 水平较高，L. reuteri 263 可能诱导 WAT 棕色化。最后，L. reuteri 263 改变了 WAT 中参与葡萄糖和脂质代谢的基因的表达，包括增加葡萄糖转运蛋白 4 和碳水化合物反应元件结合蛋白的水平，降低乙酰辅酶 A 羧化酶-1 的表达。结果表明，L. reuteri 263 可能通过高能饮食诱导肥胖大鼠 WAT 的能量代谢重塑来治疗肥胖。
