Department of Pediatrics Hematology, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao, China.
Department of Pediatrics Hematology, The Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Qingdao, China.
Biomed Pharmacother. 2018 Mar;99:96-100. doi: 10.1016/j.biopha.2018.01.042. Epub 2018 Jan 9.
Immunotherapy is important to improve the survival of children with acute lymphoblastic leukemia (ALL). This study aimed to assess the effects of crocin on the proliferation and function of T cells isolated from children with ALL.
The mononuclear cells were isolated from peripheral blood of children with ALL and then treated with different final concentrations of crocin. The levels of different cytokines secreted by T cells and the ratio of CD4 and CD8 were measured. Tail DNA% (TDNA), Tail moment (TM), Tail length (TL) and sister chromatid exchange (SCE) were detected to assess DNA damage of T cells.
Crocin significantly promoted T cell proliferation and the secretion of IL-2 and IL-4 in a concentration dependent manner. In addition, crocin increased CD4/CD8 ratio of T subset. Crocin itself caused no significant damage to T cells but reduced DNA damage in T cells treated with Ara-C.
Crocin could improve the proliferation and cytotoxic function of T cells, and reduce DNA damage caused by Ara-C.
免疫疗法对于提高急性淋巴细胞白血病（ALL）患儿的生存率非常重要。本研究旨在评估西红花酸对 ALL 患儿分离的 T 细胞增殖和功能的影响。
从 ALL 患儿外周血中分离单个核细胞，然后用不同终浓度的西红花酸处理。测量 T 细胞分泌的不同细胞因子水平和 CD4 和 CD8 的比例。检测尾 DNA%（TDNA）、尾矩（TM）、尾长（TL）和姐妹染色单体交换（SCE），以评估 T 细胞的 DNA 损伤。
西红花酸呈浓度依赖性地显著促进 T 细胞增殖和 IL-2 和 IL-4 的分泌。此外，西红花酸增加了 T 细胞亚群的 CD4/CD8 比值。西红花酸本身对 T 细胞没有明显的损伤作用，但可降低阿糖胞苷处理的 T 细胞的 DNA 损伤。
西红花酸可改善 T 细胞的增殖和细胞毒性功能，并减少阿糖胞苷引起的 DNA 损伤。
