Munem Marwa, Zaar Oscar, Dimovska Nilsson Kelly, Neittaanmäki Noora, Paoli John, Fletcher John S
Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg 412 96, Sweden.
Department of Dermatology and Venereology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg 413 45, Sweden.
Biointerphases. 2018 Jan 12;13(3):03B402. doi: 10.1116/1.5016254.
A set of basal cell carcinoma samples, removed by Mohs micrographic surgery and pathologically identified as having an aggressive subtype, have been analyzed using time-of-flight secondary ion mass spectrometry (SIMS). The SIMS analysis employed a gas cluster ion beam (GCIB) to increase the sensitivity of the technique for the detection of intact lipid species. The GCIB also allowed these intact molecular signals to be maintained while surface contamination and delocalized chemicals were removed from the upper tissue surface. Distinct mass spectral signals were detected from different regions of the tissue (epidermis, dermis, hair follicles, sebaceous glands, scar tissue, and cancerous tissue) allowing mass spectral pathology to be performed. The cancerous regions of the tissue showed a particular increase in sphingomyelin signals that were detected in both positive and negative ion mode along with increased specific phosphatidylserine and phosphatidylinositol signals observed in negative ion mode. Samples containing mixed more and less aggressive tumor regions showed increased phosphatidylcholine lipid content in the less aggressive areas similar to a punch biopsy sample of a nonaggressive nodular lesion.
一组通过莫氏显微外科手术切除并经病理鉴定为具有侵袭性亚型的基底细胞癌样本，已使用飞行时间二次离子质谱（SIMS）进行了分析。SIMS分析采用气体团簇离子束（GCIB）来提高该技术检测完整脂质种类的灵敏度。GCIB还能在从组织表面去除表面污染物和离域化学物质的同时，保持这些完整的分子信号。从组织的不同区域（表皮、真皮、毛囊、皮脂腺、瘢痕组织和癌组织）检测到了不同的质谱信号，从而能够进行质谱病理学分析。组织的癌性区域显示鞘磷脂信号显著增加，在正离子和负离子模式下均能检测到，同时在负离子模式下观察到特定的磷脂酰丝氨酸和磷脂酰肌醇信号增加。含有侵袭性程度不同的混合肿瘤区域的样本，在侵袭性较小的区域显示出磷脂酰胆碱脂质含量增加，类似于非侵袭性结节性病变的钻孔活检样本。
