De La Cruz Hernández Sergio Isaac, Anaya Molina Yazmin, Gómez Santiago Fabián, Terán Vega Heidi Lizbeth, Monroy Leyva Elda, Méndez Pérez Héctor, García Lozano Herlinda
Laboratorio de Virus Gastrointestinales, Departamento de Virología, Instituto de Diagnóstico y Referencia Epidemiológicos (InDRE), Francisco de P Miranda 177, Lomas de Plateros, 01480, Álvaro Obregón, CDMX, Mexico.
Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional (ENCB-IPN), Prolongación de Carpio y Plan de Ayala s/n, Santo Tomás, 11340, Miguel Hidalgo, CDMX, Mexico.
Diagn Microbiol Infect Dis. 2018 Apr;90(4):272-276. doi: 10.1016/j.diagmicrobio.2017.12.005. Epub 2017 Dec 14.
Rotavirus produces diarrhea in children under 5 years old. Most of those conventional methods such as polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT-PCR) have been used for rotavirus detection. However, these techniques need a multi-step process to get the results. In comparison with conventional methods, the real-time RT-PCR is a highly sensitive method, which allows getting the results in only one day. In this study a real-time RT-PCR assay was tested using a panel of 440 samples from patients with acute gastroenteritis, and characterized by PAGE and RT-PCR. The results show that the real-time RT-PCR detected rotavirus from 73% of rotavirus-negative samples analyzed by PAGE and RT-PCR; thus, the percentage of rotavirus-positive samples increased to 81%. The results indicate that this real-time RT-PCR should be part of a routine analysis, and as a support of the diagnosis of rotavirus in Mexico.
轮状病毒会导致5岁以下儿童腹泻。诸如聚丙烯酰胺凝胶电泳（PAGE）和逆转录-聚合酶链反应（RT-PCR）等大多数传统方法已用于轮状病毒检测。然而，这些技术需要多步骤才能获得结果。与传统方法相比，实时RT-PCR是一种高度灵敏的方法，仅需一天就能获得结果。在本研究中，使用一组来自急性肠胃炎患者的440份样本对实时RT-PCR检测法进行了测试，并通过PAGE和RT-PCR进行了特征分析。结果显示，实时RT-PCR从经PAGE和RT-PCR分析的73%轮状病毒阴性样本中检测到了轮状病毒；因此，轮状病毒阳性样本的百分比增至81%。结果表明，这种实时RT-PCR应成为常规分析的一部分，并作为墨西哥轮状病毒诊断的辅助手段。
