Wu Jin-Yuan, Zhou Yan, Zhang Guang-Ming, Mu Guo-Fa, Yi Shan, Yin Na, Xie Yu-Ping, Lin Xiao-Chen, Li Hong-Jun, Sun Mao-Sheng
Department of Molecular Biology, Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, Yunnan Province, China.
Pediatrics Department, the First People's Hospital of Zhaotong City, Zhaotong 657000, Yunnan Province, China.
World J Clin Cases. 2018 Oct 6;6(11):426-440. doi: 10.12998/wjcc.v6.i11.426.
To determine the distribution of rotavirus gene in hospitalized children in Yunnan, China.
A total of 366 stool specimens were collected from hospitalized children in hospitals in Yunnan Province from September 2010 to December 2013. The genomic RNA electropherotypes and the G genotypes of the rotaviruses were determined. A phylogenetic analysis of the gene was performed. Rotavirus isolation was performed, and characterized by plaque, minimum essential medium, and all genes sequence analysis. Quantification of antibodies for inactivated vaccine prepared with ZTR-68 was examined by enzyme-linked immunosorbent assay and microneutralization assay.
Group A human rotavirus was detected in 177 of 366 (48.4%) stool samples using a colloidal gold device assay. The temporal distribution of rotavirus cases showed significant correlation with the mean air temperature. Rotaviruses were isolated from 13% of the rotavirus-positive samples. The predominant genotype was G1 (43.5%), followed by G3 (21.7%), G9 (17.4%), G2 (4.3%), G4 (8.7%), and mixed (4.3%) among a total of 23 rotavirus isolates. A rotavirus strain was isolated from a rotavirus-positive stool sample of a 4-month-old child in The First People's Hospital of Zhaotong (2010) for use as a candidate human inactivated rotavirus vaccine strain and for further research, and was designated ZTR-68. The genotype of 11 gene segments of strain ZTR-68 (RVA/Human-wt/CHN/ZTR-68/2010/G1P[8]) was characterized. The genotype constellation of strain ZTR-68 was identified as G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. The and genotypes of strain ZTR-68 were similar to Wa-like strains.CONCLUSIONSA high prevalence of the G1, G2, and G3 genotypes was detected from 2010 to 2012. However, a dominant prevalence of the G9 genotype was identified as the cause of gastroenteritis in children in Yunnan, China, in 2013. A candidate human inactivated rotavirus vaccine strain, designated ZTR-68 was isolated, characterized, and showed immunogenicity. Our data will be useful for the future formulation and development of a vaccine in China.
确定中国云南住院儿童中轮状病毒基因的分布情况。
2010年9月至2013年12月期间，从云南省各医院的住院儿童中收集了366份粪便标本。测定轮状病毒的基因组RNA电泳图谱和G基因型。对该基因进行系统发育分析。进行轮状病毒分离，并通过蚀斑、最低必需培养基和所有基因序列分析进行鉴定。采用酶联免疫吸附测定法和微量中和试验检测用ZTR-68制备的灭活疫苗的抗体定量。
使用胶体金检测法在366份粪便样本中的177份（48.4%）中检测到A组人轮状病毒。轮状病毒病例的时间分布与平均气温显示出显著相关性。从13%的轮状病毒阳性样本中分离出轮状病毒。在总共23株轮状病毒分离株中，主要基因型为G1（43.5%），其次是G3（21.7%）、G9（17.4%）、G2（4.3%）、G4（8.7%）和混合基因型（4.3%）。从昭通市第一人民医院（2010年）一名4个月大儿童的轮状病毒阳性粪便样本中分离出一株轮状病毒株，用作候选人用灭活轮状病毒疫苗株并进行进一步研究，命名为ZTR-68。对ZTR-68株（RVA/Human-wt/CHN/ZTR-68/2010/G1P[8]）的11个基因片段的基因型进行了鉴定。ZTR-68株的基因型组合被鉴定为G1-P[8]-I1-R1-C1-M1-A1-N1-TI-E1-H1。ZTR-68株的 和 基因型与Wa样毒株相似。
2010年至2012年期间检测到G1、G2和G3基因型的高流行率。然而，2013年在中国云南，G9基因型的优势流行被确定为儿童胃肠炎的病因。分离、鉴定了一株候选人用灭活轮状病毒疫苗株ZTR-68，并显示出免疫原性。我们的数据将有助于中国未来疫苗的配方制定和开发。
