Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India.
Centre for Lipid Research, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad 500007, India.
Food Chem. 2018 May 15;248:210-216. doi: 10.1016/j.foodchem.2017.12.066. Epub 2017 Dec 19.
An enzymatic process was developed for the preparation of a nutritionally enriched 1,3-diacylglycerol(DAG)-rich oil from a blend of refined sunflower and rice bran oils. The process involves hydrolysis of vegetable oil blend using Candida cylindracea followed by esterification with glycerol using Lipozyme RM1M. The resultant DAG-rich oil contains 84% of DAG (66% of 1,3-DAG, 18% of 1,2-DAG) and 16% of triacylglycerol (TAG) along with micro nutrients like γ-oryzanol, tocotrienols, tocopherols and phytosterols. Nutritional studies of the DAG-rich oil were conducted in Wistar rats and compared with sunflower oil (SFO). The calorific value of the DAG-rich oil was estimated to be 6.45 Kcals/g as against 9.25 Kcals/g for SFO. The serum and liver cholesterol and TAG levels in rats fed with 1,3-DAG-rich oil were found to be significantly reduced as compared to rats fed diet containing SFO. We conclude that 1,3-DAG-rich oil is a low calorie fat and exhibits hypolipidemic effects.
开发了一种酶法工艺，从精制葵花籽油和米糠油的混合物中制备富含营养的 1,3-二酰基甘油（DAG）的油。该工艺包括使用 Candida cylindracea 水解植物油混合物，然后使用 Lipozyme RM1M 用甘油进行酯化。所得富含 DAG 的油含有 84%的 DAG（66%的 1,3-DAG，18%的 1,2-DAG）和 16%的三酰基甘油（TAG）以及微量营养素，如γ-谷维素、生育三烯酚、生育酚和植物甾醇。在 Wistar 大鼠中进行了富含 DAG 的油的营养研究，并与葵花籽油（SFO）进行了比较。富含 DAG 的油的热值估计为 6.45 Kcals/g，而 SFO 的热值为 9.25 Kcals/g。与喂食含有 SFO 的饮食的大鼠相比，喂食富含 1,3-DAG 的油的大鼠的血清和肝脏胆固醇和 TAG 水平显着降低。我们得出的结论是，富含 1,3-DAG 的油是一种低卡路里的脂肪，具有降低血脂的作用。
