JNU-UPM International Joint Laboratory on Plant Oil Processing and Safety, Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China.
College of Pharmacy, Jinan University, Guangzhou 510632, China.
Nutrients. 2024 Jun 24;16(13):2003. doi: 10.3390/nu16132003.
To investigate the effects of rapeseed diacylglycerol oil (RDG) intake on lipid accumulation and metabolism in C57BL/6J mice, obese mice were fed a high-fat diet in which 45% of the total energy content came from RDG (RDGM group) or rapeseed triacylglycerol oil (RTGM group). This diet intervention was conducted for 12 weeks following the establishment of the obese mouse model. By the end of the experiment, the serum glucose levels of the mice in the RTGM and RDGM groups were 13.0 ± 1.3 mmol/L and 9.7 ± 1.5 mmol/L, respectively. Meanwhile, the serum triglyceride level in the RDGM group was 26.3% lower than that in the RTGM group. The weight-loss effect in the RDGM group was accompanied by a significant decrease in the white adipose tissue (WAT) index. The RDG intervention did not significantly change the antioxidant and anti-inflammatory properties of the rapeseed oil in vivo. The RDG diet improved the liver lipid metabolism abnormalities induced by a high-fat diet, leading to decreased liver damage index values (AST and ALT). Additionally, compared to that in the RTGM group, the expression of the adipogenic genes and decreased in both the liver and intestine by 21.7% and 16.7% and by 38.7% and 47.2%, respectively, in the RDGM group. Further, most lipolytic genes in BAT showed no significant change after the RDG intervention. This implies that RDG regulates lipid metabolism by altering the expression of adipogenic genes in the liver, intestine, and adipose tissue, thereby reducing the accumulation of WAT. Furthermore, the RDG diet enhanced gut flora diversity, increasing the relative levels of and decreasing the levels of and in the mouse gut, potentially accelerating lipid metabolism. Thus, a three-month RDG diet intervention in obese mice exhibited benefits in regulating the somatotype, serum obesity-related indices, gut flora structure, and lipid metabolism in the adipose tissue, liver, and intestine.
为了研究菜籽油二酰基甘油（RDG）摄入对 C57BL/6J 小鼠脂积累和代谢的影响，肥胖小鼠喂食高脂肪饮食，其中 45%的总能量来自 RDG（RDGM 组）或菜籽油三酰基甘油（RTGM 组）。在肥胖小鼠模型建立后，进行了为期 12 周的饮食干预。实验结束时，RTGM 和 RDGM 组小鼠的血清葡萄糖水平分别为 13.0±1.3mmol/L 和 9.7±1.5mmol/L，同时 RDGM 组血清甘油三酯水平比 RTGM 组低 26.3%。RDGM 组的减肥效果伴随着白色脂肪组织（WAT）指数的显著降低。RDG 干预并没有显著改变体内菜籽油的抗氧化和抗炎特性。RDG 饮食改善了高脂肪饮食引起的肝脏脂质代谢异常，导致肝损伤指数值（AST 和 ALT）降低。此外，与 RTGM 组相比，RDGM 组肝脏和肠道中脂肪生成基因 和 的表达分别降低了 21.7%和 16.7%，以及 38.7%和 47.2%。此外，BAT 中的大多数脂肪分解基因在 RDG 干预后没有明显变化。这表明 RDG 通过改变肝脏、肠道和脂肪组织中脂肪生成基因的表达来调节脂质代谢，从而减少 WAT 的积累。此外，RDG 饮食增强了肠道菌群的多样性，增加了 和减少了 和 在小鼠肠道中的相对水平，可能加速了脂质代谢。因此，肥胖小鼠三个月的 RDG 饮食干预在调节体型、血清肥胖相关指标、肠道菌群结构和脂肪组织、肝脏和肠道的脂质代谢方面表现出益处。
