Muñoz-Cabello Ana María, Torres-Torrelo Hortensia, Arias-Mayenco Ignacio, Ortega-Sáenz Patricia, López-Barneo José
Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
Departamento de Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, Seville, Spain.
Methods Mol Biol. 2018;1742:125-137. doi: 10.1007/978-1-4939-7665-2_12.
The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluorimetry and the patch-clamp technique.
颈动脉体是哺乳动物主要的动脉化学感受器，介导由急性缺氧激活的心肺反射。在此，我们描述了我们实验室所采用的实验方案，用于研究通过微量荧光测定法和膜片钳技术监测的单个酶分散球细胞对缺氧的反应性。
