Ortega-Sáenz Patricia, Caballero Candela, Gao Lin, López-Barneo José
Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla, Seville, Spain.
Departamento de Fisiología Médica y Biofísica, Facultad de Medicina, Universidad de Sevilla, Seville, Spain.
Methods Mol Biol. 2018;1742:139-153. doi: 10.1007/978-1-4939-7665-2_13.
Monitoring responsiveness to acute hypoxia of whole animals and single cells is essential to investigate the nature of the mechanisms underlying oxygen (O) sensing. Here we describe the protocols followed in our laboratory to evaluate the ventilatory response to hypoxia in normal and genetically modified animals. We also describe the amperometric technique used to monitor single-cell catecholamine release from chemoreceptor cells in carotid body and adrenal medulla slices.
监测整个动物和单细胞对急性缺氧的反应性对于研究氧（O）感知潜在机制的本质至关重要。在此，我们描述了我们实验室用于评估正常和转基因动物对缺氧通气反应所遵循的方案。我们还描述了用于监测来自颈动脉体和肾上腺髓质切片中化学感受器细胞的单细胞儿茶酚胺释放的安培测量技术。
