将反义吗啉代寡核苷酸、CRISPR/Cas9核糖核蛋白以及RNA/DNA显微注射到斑马鱼胚胎中。
Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos.
作者信息
Xin Yi, Duan Cunming
机构信息
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
出版信息
Methods Mol Biol. 2018;1742:205-211. doi: 10.1007/978-1-4939-7665-2_18.
PMID:29330802
Abstract
In this chapter, we describe a stepwise protocol of microinjection. Using this method, antisense morpholinos, CRISPR-Cas9 ribonucleoprotein complexes, capped mRNA, and DNA can be delivered into fertilized zebrafish eggs to manipulate gene expression during development. This protocol can also be adapted for microinjection in other fish and amphibian species.
摘要
在本章中，我们描述了一种显微注射的逐步方案。使用这种方法，可以将反义吗啉代寡核苷酸、CRISPR-Cas9核糖核蛋白复合物、加帽mRNA和DNA注入受精的斑马鱼卵中，以在发育过程中操纵基因表达。该方案也可适用于其他鱼类和两栖类物种的显微注射。
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