Department of Biosciences, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy.
Department of Biosciences, Università degli Studi di Milano, Via Celoria 26, 20133 Milano, Italy.
Trends Plant Sci. 2018 Apr;23(4):293-301. doi: 10.1016/j.tplants.2017.12.005. Epub 2018 Jan 10.
CONSTANS (CO) is a master regulator of flowering time, although the mechanisms underlying its role as a transcriptional regulator are not well understood. The DNA-binding domain of CO shares homology with that of NUCLEAR FACTOR YA (NF-YA), a subunit of the CCAAT-binding trimer NF-Y. Recent publications indicate that CO and its rice homolog HEADING DATE 1 (Hd1) form heterotrimers with the histone-fold subunits of NF-Y to efficiently bind promoter elements in the florigen genes. Differences in the DNA-binding specificities of NF-Y and NF-CO can be conceptualized based on our knowledge of the 3D structure of the NF-Y/CCAAT complex. Here we discuss the modes of assembly of NF-Y-like heterotrimers and possible models for their activity as flexible sequence-specific transcriptional regulators.
CONSTANS（CO）是开花时间的主要调节剂，尽管其作为转录调节剂的作用机制尚不清楚。CO 的 DNA 结合域与核因子 YA（NF-YA）同源，NF-YA 是 CCAAT 结合三聚体 NF-Y 的一个亚基。最近的出版物表明，CO 和它的水稻同源物Heading Date 1（Hd1）与 NF-Y 的组蛋白折叠亚基形成异源三聚体，以有效地结合花生成基因中的启动子元件。根据我们对 NF-Y/CCAAT 复合物 3D 结构的了解，可以将 NF-Y 和 NF-CO 的 DNA 结合特异性的差异概念化。在这里，我们讨论了 NF-Y 样异源三聚体的组装方式及其作为灵活的序列特异性转录调节剂的可能模型。
