Zhang Pingxian, Li Xiulan, Wang Yifan, Guo Weijun, Chachar Sadaruddin, Riaz Adeel, Geng Yuke, Gu Xiaofeng, Yang Liwen
Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081 China.
College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 Hubei China.
aBIOTECH. 2021 Dec 2;2(4):403-414. doi: 10.1007/s42994-021-00065-y. eCollection 2021 Dec.
The timing of floral transition is critical for reproductive success in flowering plants. In long-day (LD) plant , the floral regulator gene () is a major component of the mobile florigen. expression is rhythmically activated by CONSTANS (CO), and specifically accumulated at dusk of LDs. However, the underlying mechanism of adequate regulation of transcription in response to day-length cues to warrant flowering time still remains to be investigated. Here, we identify a homolog of human protein arginine methyltransferases 6 (HsPRMT6) in , and confirm AtPRMT6 physically interacts with three positive regulators of flowering Nuclear Factors YC3 (NF-YC3), NF-YC9, and NF-YB3. Further investigations find that and its encoding protein accumulate at dusk of LDs. PRMT6-mediated H3R2me2a modification enhances the promotion of NF-YCs on transcription in response to inductive LD signals. Moreover, AtPRMT6 and its homologues proteins AtPRMT4a and AtPRMT4b coordinately inhibit the expression of , a suppressor of . Taken together, our study reveals the role of arginine methylation in photoperiodic pathway and how the PRMT6-mediating H3R2me2a system interacts with NF-CO module to dynamically control expression and facilitate flowering time.
The online version contains supplementary material available at 10.1007/s42994-021-00065-y.
花转变的时间对于开花植物的繁殖成功至关重要。在长日照（LD）植物中，花调控基因（）是移动成花素的主要成分。的表达由CONSTANS（CO）有节奏地激活，并在长日照的黄昏时特异性积累。然而，响应日长信号以确保开花时间而对转录进行充分调控的潜在机制仍有待研究。在这里，我们在中鉴定出人类蛋白质精氨酸甲基转移酶6（HsPRMT6）的同源物，并证实AtPRMT6与开花核因子YC3（NF-YC3）、NF-YC9和NF-YB3这三个正向调控因子发生物理相互作用。进一步研究发现和其编码蛋白在长日照的黄昏时积累。PRMT6介导的H3R2me2a修饰增强了NF-YCs在响应诱导性长日照信号时对转录的促进作用。此外，AtPRMT6及其同源蛋白AtPRMT4a和AtPRMT4b协同抑制的表达，是一种的抑制因子。综上所述，我们的研究揭示了精氨酸甲基化在光周期途径中的作用，以及PRMT6介导的H3R2me2a系统如何与NF-CO模块相互作用以动态控制表达并促进开花时间。
在线版本包含可在10.1007/s42994-021-00065-y获取的补充材料。
