Augustine Dominic, Rao Roopa S, Anbu Jayaraman, Chidambara Murthy K N
Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, M. S. Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.
Department of Pharmacology, Faculty of Pharmacy, M. S. Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.
Pharmacognosy Res. 2017 Dec;9(Suppl 1):S61-S66. doi: 10.4103/pr.pr_52_17.
The earthworm coelomic fluid (ECF) has shown proven antiproliferative effect against breast, liver, gastrointestinal, and brain cancer, but it is least explored in oral cancer. The present study is an attempt to investigate the antiproliferative activity of ECF on oral cancer cell line squamous cell carcinoma (SCC)-9.
ECF was collected from the species (EE), (EF), and (PE) stored at -80°C. Percentage inhibition of ECF on squamous cell carcinoma-9 cells was recorded at 24 h. Protein estimation was done using Bradford protein assay validated by the biuret method. Cytotoxicity was tested at 2.5, 5, 10, 20, 40, and 80 μg/ml concentrations by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay in SCC-9 cells . GraphPad Prism 7.0 software was used to calculate the inhibitory concentration (IC). Chi-square test was used to analyze the difference between samples.
The test samples EE, EF, and PE inhibited the growth of SCC-9 cells significantly in a dose-dependent manner, and the IC values were found to be 4.6, 44.69, and 5.27 μg/ml, respectively. The antiproliferative effect was found to be variable among the three earthworm species with EE showing the most promising effect followed by PE and EF.
Establishing the antiproliferative effect of ECF on oral cancer cells could be an initial step toward drug development and future anticancer research. The preliminary investigation has shown that ECF has a promising antiproliferative effect on oral cancer cells .
The present pilot study evaluated the antiproliferative effect of earthworm coelomic fluid (ECF) of (EE), (EF), and (PE) on squamous cell carcinoma-9 cell line. The ECF inhibitory activity was promising at inhibitory concentration values of 4.6, 44.69, and 5.27 μg/ml, respectively. Further studies pertaining to antiproliferative mechanism of EE, EF, and PE have been planned. ECF: Earthworm coelomic fluid, EE: , EF: , PE: , MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, SCC: Squamous cell carcinoma, BSA: Bovine serum albumin, PBS: Phosphored buffered saline, ATCC: American Type Culture Collection.
蚯蚓体腔液（ECF）已被证明对乳腺癌、肝癌、胃肠道癌和脑癌具有抗增殖作用，但在口腔癌方面的研究最少。本研究旨在探讨ECF对口腔癌细胞系鳞状细胞癌（SCC）-9的抗增殖活性。
从储存于-80°C的（EE）、（EF）和（PE）品种蚯蚓中收集ECF。记录24小时时ECF对鳞状细胞癌-9细胞的抑制百分比。蛋白质定量采用经双缩脲法验证的Bradford蛋白质测定法。通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐法在SCC-9细胞中以2.5、5、10、20、40和80μg/ml的浓度测试细胞毒性。使用GraphPad Prism 7.0软件计算抑制浓度（IC）。采用卡方检验分析样本间差异。
测试样本EE、EF和PE均以剂量依赖性方式显著抑制SCC-9细胞的生长，IC值分别为4.6、44.69和5.27μg/ml。三种蚯蚓品种的抗增殖作用存在差异，EE表现出最显著的效果，其次是PE和EF。
确定ECF对口腔癌细胞的抗增殖作用可能是药物开发和未来抗癌研究的第一步。初步研究表明，ECF对口腔癌细胞具有显著的抗增殖作用。
本初步研究评估了（EE）、（EF）和（PE）的蚯蚓体腔液（ECF）对鳞状细胞癌-9细胞系的抗增殖作用。ECF的抑制活性分别在4.6、44.69和5.27μg/ml的抑制浓度值时表现出前景。已计划对EE、EF和PE的抗增殖机制进行进一步研究。ECF：蚯蚓体腔液，EE： ，EF： ，PE： ，MTT：3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐，SCC：鳞状细胞癌，BSA：牛血清白蛋白，PBS：磷酸盐缓冲盐水，ATCC：美国典型培养物保藏中心
