Shin Eung Jin, Kim Han Jo, Son Myoung Won, Ahn Tae Sung, Lee Hyun Yong, Lim Dae Ro, Bae Sang Byung, Jeon Seob, Kim Hyungjoo, Jeong Dongjun, Lee Moon Soo, Kim Dong-Sun, Noh Jeong Se, Baek Moo-Jun
Department of Surgery, Soonchunhyang University College of Medicine, Cheonan, Korea.
Department of Hematology and Oncology, Soonchunhyang University College of Medicine, Cheonan, Korea.
Ann Surg Treat Res. 2018 Jan;94(1):19-25. doi: 10.4174/astr.2018.94.1.19. Epub 2017 Dec 28.
Emerging evidence indicates that runt-related transcription factor 3 () is an important tumor suppressor gene in several cancer types, including colorectal cancer (CRC). However, the clinical significance of RUNX3 inactivation in CRC remains unclear. The aim of this study was to examine the correlation between clinicopathologic factors and RUNX3 hypermethylation/expression in CRC.
Sixty-two CRC patients who were treated at the Soonchunhyang University College of Medicine were recruited in this study. The hypermethylation of CpG islands in the RUNX3 promoter and the expression of RUNX3 mRNA were identified by methylation-specific polymerase chain reaction (PCR) and reverse transcriptase-PCR, respectively. The expression of RUNX3 was determined by immunohistochemical staining.
Of the 62 CRC tissue samples, 20 (32.3%) presented hypermethylated RUNX3 promoters. Aberrant RUNX3 hypermethylation was found to be associated with vascular (P = 0.006) and lymphatic (P = 0.002) invasion. Hypermethylation of RUNX3 was associated with poor survival outcomes (P = 0.038). However, expression of RUNX3 was not a prognostic factor (P = 0.363).
Hypermethylation of RUNX3 may be a predictor of a poor prognosis in CRC.
新出现的证据表明， runt相关转录因子3（RUNX3）在包括结直肠癌（CRC）在内的多种癌症类型中是一种重要的肿瘤抑制基因。然而，RUNX3失活在CRC中的临床意义仍不清楚。本研究的目的是探讨CRC中临床病理因素与RUNX3高甲基化/表达之间的相关性。
本研究招募了62例在顺天乡大学医学院接受治疗的CRC患者。分别通过甲基化特异性聚合酶链反应（PCR）和逆转录PCR鉴定RUNX3启动子中CpG岛的高甲基化和RUNX3 mRNA的表达。通过免疫组织化学染色确定RUNX3的表达。
在62例CRC组织样本中，20例（32.3%）呈现RUNX3启动子高甲基化。发现异常的RUNX3高甲基化与血管侵犯（P = 0.006）和淋巴侵犯（P = 0.002）相关。RUNX3高甲基化与不良生存结果相关（P = 0.038）。然而，RUNX3的表达不是一个预后因素（P = 0.363）。
RUNX3高甲基化可能是CRC预后不良的一个预测指标。
