Vecchi Joseph T, Wakatsuki Tetsuro
InvivoSciences, Inc., USA.
Arch Stem Cell Res. 2015;3(1). Epub 2016 Nov 8.
Pluripotent Stem Cells were originally derived and cultured using a feeder layer of cells. Movements have been undertaken to transition from this method to one more defined, high-throughput, and without xenogenic factors. Tremendous research has been done in this area and many products have been developed, however, based on our analysis of recent publications in stem cell related journals many in academia are still using older methods like a feeder layer. In this short communication, we discuss the feasibility of transitioning to defined, xeno-free methods, how a standardized method could improve the field and industry, and that a study bringing together multiple institutions comparing culture methods could be done to evaluate the efficacy of these new methods.
多能干细胞最初是使用饲养层细胞进行衍生和培养的。目前已开展了相关行动，旨在从这种方法过渡到一种定义更明确、高通量且无外源因素的方法。该领域已开展了大量研究并开发了许多产品，然而，基于我们对干细胞相关期刊近期出版物的分析，学术界许多人仍在使用诸如饲养层等较老的方法。在这篇简短的通讯中，我们讨论了过渡到定义明确的无外源方法的可行性、标准化方法如何能改进该领域和行业，以及可以开展一项汇集多个机构比较培养方法的研究，以评估这些新方法的有效性。
