LISBP, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.
Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Bethesda, Maryland 20892-0520, USA.
Analyst. 2018 Feb 7;143(3):620-629. doi: 10.1039/c7an01635b. Epub 2018 Jan 15.
In-cell NMR of macromolecules has gained momentum over the last ten years as an approach that might bridge the branches of cell biology and structural biology. In this review, we put it in the context of earlier efforts that aimed to characterize by NMR the cellular environment of live cells and their intracellular metabolites. Although technical aspects distinguish these earlier in vivo NMR studies and the more recent in cell NMR efforts to characterize macromolecules in a cellular environment, we believe that both share major concerns ranging from sensitivity and line broadening to cell viability. Approaches to overcome the limitations in one subfield thereby can serve the other one and vice versa. The relevance in biomedical sciences might stretch from the direct following of drug metabolism in the cell to the observation of target binding, and thereby encompasses in-cell NMR both of metabolites and macromolecules. We underline the efforts of the field to move to novel biological insights by some selected examples.
在过去十年中，细胞内 NMR 作为一种可能连接细胞生物学和结构生物学分支的方法得到了发展。在这篇综述中，我们将其置于早期旨在通过 NMR 表征活细胞及其细胞内代谢物的细胞环境的努力的背景下。尽管技术方面将这些早期的体内 NMR 研究与最近的细胞内 NMR 努力区分开来，以在细胞环境中表征大分子，但我们认为两者都存在从灵敏度和线宽到细胞活力的主要关注点。克服一个子领域限制的方法因此可以为另一个领域服务，反之亦然。在生物医学科学中的相关性可能从细胞内药物代谢的直接跟踪延伸到靶标结合的观察，因此细胞内 NMR 既包括代谢物又包括大分子。我们通过一些选定的例子强调了该领域在获得新的生物学见解方面所做的努力。
