Huang Rong, Jiang Bo-Guang, Li Xiao-Nian, Wang Ya-Ting, Liu Si-Si, Zheng Kai-Xuan, He Jian, Wu Shao-Hua
School of Chemical Science and Technology, Yunnan University , Kunming 650091, China.
Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University , Kunming 650091, China.
J Agric Food Chem. 2018 Feb 7;66(5):1140-1146. doi: 10.1021/acs.jafc.7b04998. Epub 2018 Jan 30.
Seven new polyoxygenated cyclohexenoids, namely, phomopoxides A-G (1-7), were isolated from the fermentation broth extract of an endophytic fungal strain Phomopsis sp. YE3250 from the medicinal plant Paeonia delavayi Franch. The structures of these compounds were established by spectroscopic interpretation. The absolute configurations of compounds 1 and 4 were confirmed by X-ray crystallographic analysis and chemical derivative approach. All isolated compounds showed weak cytotoxic activities toward three human tumor cell lines (Hela, MCF-7, and NCI-H460) and weak antifungal activities against five pathogenic fungi (Candida albicans, Aspergillus niger, Pyricularia oryzae, Fusarium avenaceum, and Hormodendrum compactum). In addition, compounds 1-7 showed a promising α-glycosidase inhibitory activity with IC values of 1.47, 1.55, 1.83, 2.76, 2.88, 3.16, and 2.94 mM, respectively, as compared with a positive control of acarbose (IC = 1.22 mM).
从药用植物滇牡丹（Paeonia delavayi Franch.）的内生真菌菌株拟茎点霉（Phomopsis sp.）YE3250的发酵液提取物中分离得到7个新的多氧环己烯类化合物，即拟茎点霉氧化物A - G（1 - 7）。通过光谱解析确定了这些化合物的结构。化合物1和4的绝对构型通过X射线晶体学分析和化学衍生方法得以确证。所有分离得到的化合物对三种人类肿瘤细胞系（Hela、MCF - 7和NCI - H460）均表现出较弱的细胞毒性活性，对五种致病真菌（白色念珠菌、黑曲霉、稻瘟病菌、燕麦镰刀菌和紧密单孢枝霉）也表现出较弱的抗真菌活性。此外，与阳性对照阿卡波糖（IC = 1.22 mM）相比，化合物1 - 7表现出良好的α - 糖苷酶抑制活性，IC值分别为1.47、1.55、1.83、2.76、2.88、3.16和2.94 mM。
