Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission & Ministry of Education, Yunnan University of Nationalities, Kunming 650031, PR China.
Clinical Laboratory, First People's Hospital of Yunnan Province, Kunming 650032, PR China.
Fitoterapia. 2013 Dec;91:189-193. doi: 10.1016/j.fitote.2013.09.004. Epub 2013 Sep 13.
Three new xanthones, 1,5-dihydroxy-3-hydroxyethyl-6-methoxycarbonylxanthone (1), 1-hydroxy-5- methoxy-3-hydroxyethyl-6-methoxycarbonylxanthone (2), and 1-hydroxy-3-hydroxyethyl- 8-ethoxycarbonylxanthone (3), along with seven known xanthones (4-10) were isolated from the fermentation products of an endophytic fungus Phomopsis sp.. Their structures were elucidated by spectroscopic methods including extensive 1D- and 2D-NMR techniques. Compounds 1-10 were also tested for their cytotoxicity against five human tumor cell lines (NB4, A549, SHSY5Y, PC3, and MCF7) by MTT method using paclitaxel as positive control. Compounds 1 and 3 showed cytotoxicity against A549 cell lines with IC50 values of 3.6 and 2.5 μM, respectively. In addition, 1 was cytotoxic to MCF7 cells with IC50 value of 2.7 μM.
从内生真菌 Phomopsis sp. 的发酵产物中分离得到了三种新的紫檀烷酮，分别为 1,5-二羟基-3-羟乙基-6-甲氧基羰基紫檀烷酮（1）、1-羟基-5-甲氧基-3-羟乙基-6-甲氧基羰基紫檀烷酮（2）和 1-羟基-3-羟乙基-8-乙氧羰基紫檀烷酮（3），以及七种已知的紫檀烷酮（4-10）。通过包括广泛的 1D-和 2D-NMR 技术在内的光谱方法阐明了它们的结构。采用 MTT 法，以紫杉醇为阳性对照，用五种人肿瘤细胞系（NB4、A549、SHSY5Y、PC3 和 MCF7）对化合物 1-10 进行了细胞毒性测试。化合物 1 和 3 对 A549 细胞系表现出细胞毒性，IC50 值分别为 3.6 和 2.5 μM。此外，化合物 1 对 MCF7 细胞系的 IC50 值为 2.7 μM。
