环状 RNA 在卵巢子宫内膜异位症中的表达。
Circular RNA expression in ovarian endometriosis.
机构信息
Department of Obstetrics & Gynecology, Xiangya Hospital, Central South University, No 87 Xiangya Road, Changsha Hunan 410008, PR China.
出版信息
Epigenomics. 2018 May;10(5):559-572. doi: 10.2217/epi-2017-0079. Epub 2018 Jan 16.
AIM
Circular RNAs (circRNAs) with miRNA response elements (MREs) could function as competing endogenous RNA (ceRNA) in regulating gene expression. This study was carried out to identify the expression profile and role of circRNAs in endometriosis.
MATERIALS & METHODS: Microarray assay was performed in four paired ovarian endometriomas and eutopic endometrium, followed by quantitative real-time RT-PCR in 24 paired samples. Bioinformatical algorithms were used to predict MREs, as well as ceRNA and KEGG pathway analysis.
RESULTS
We identified 262 upregulated and 291 downregulated circRNAs, binding with 1225 MREs. The ceRNA network included 122 miRNAs and 137 mRNAs, which are involed in nine pathways.
CONCLUSION
CircRNAs are differentially expressed in endometriosis, which might be related with pathogenesis of endometriosis.
目的
具有 miRNA 反应元件 (MRE) 的环状 RNA (circRNA) 可以作为竞争性内源 RNA (ceRNA) 调节基因表达。本研究旨在鉴定环状 RNA 在子宫内膜异位症中的表达谱和作用。
材料与方法
在四对卵巢子宫内膜异位症和在位子宫内膜中进行微阵列分析，然后在 24 对样本中进行定量实时 RT-PCR。生物信息学算法用于预测 MRE 以及 ceRNA 和 KEGG 通路分析。
结果
我们鉴定出 262 个上调和 291 个下调的 circRNA，与 1225 个 MRE 结合。ceRNA 网络包括 122 个 miRNA 和 137 个 mRNA，涉及 9 条通路。
结论
circRNA 在子宫内膜异位症中表达差异，可能与子宫内膜异位症的发病机制有关。
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