Petroff O A, Prichard J W, Behar K L, Rothman D L, Alger J R, Shulman R G
Neurology. 1985 Dec;35(12):1681-8. doi: 10.1212/wnl.35.12.1681.
Paralyzed rabbits ventilated with an oxygen, nitrous oxide, and carbon dioxide mixture were subjected to hyper- and hypocarbic stress. An Oxford Instrument TMR 32-200 spectrometer was used to record phosphorus-31 and nonwater proton nuclear magnetic resonance spectra of the in vivo brain. These spectra provide measurements of cerebral pHi, phosphocreatine, orthophosphate, ATP, and lactate. The brain exhibited twice as much acute pH-regulating ability as the arterial blood. During hypercarbia, orthophosphate rose while phosphocreatine declined in a reciprocal manner, and ATP remained constant. During hypocarbia, lactate rose gradually over a period of 1 hour, while orthophosphate, phosphocreatine, and ATP remained constant and calculated values of adenosine mono- and diphosphate rose.
用氧气、一氧化二氮和二氧化碳混合物进行通气的瘫痪兔子遭受了高碳酸血症和低碳酸血症应激。使用牛津仪器TMR 32 - 200光谱仪记录体内大脑的磷 - 31和非水质子核磁共振光谱。这些光谱可测量脑内酸碱度（pHi）、磷酸肌酸、正磷酸盐、三磷酸腺苷（ATP）和乳酸。大脑的急性pH调节能力是动脉血的两倍。在高碳酸血症期间，正磷酸盐上升，而磷酸肌酸以相反的方式下降，ATP保持恒定。在低碳酸血症期间，乳酸在1小时内逐渐上升，而正磷酸盐、磷酸肌酸和ATP保持恒定，并且单磷酸腺苷和二磷酸腺苷的计算值上升。
