Faucz Luciana L, Will Sonia E, Rodrigues Consuelo J, Hesse Henrique, Moraes Angelina C, Maria Durvanei A
Laboratory of Biochemistry and Biophysics of Butantan Institute, University of Sao Paulo, Butantã, Sao Paulo, Brazil.
Dermatology Service, Faculty of Medicine, Hospital Universitário Evangelico de Curitiba; Faculdade Evangélica do Paraná, Curitiba, Brazil.
Lasers Surg Med. 2018 Jan 16. doi: 10.1002/lsm.22782.
The aging of human skin includes intrinsic aging and photo-aging, which are characterized by a decrease in collagen and the deposition of abnormal elastic fibers. Intense pulsed light (IPL) sources are widely used in medicine to treat various cosmetic problems, including photo-damaged skin. Few studies have examined the microscopic changes produced by IPL. The objective of this study was to quantitatively evaluate the effects of IPL on collagen and elastic fibers in mice.
Forty female BALB/c mice were divided into four subgroups. Group 1 was the control group (n = 10), and groups 2, 3, and 4 were treatment groups (n = 10 in each group). Group 2 received one treatment, group 3 received two treatments, and group 4 received three treatments every 2 weeks. Skin tissue was obtained from irradiated areas 24 hours after the last treatment in each mouse. Collagen fibers were identified using the picrosirius red method. Elastic fibers were marked by Weigert-oxone stain. All samples were analyzed and quantified by a light microscope using analyzer system images.
Group 4, which received three IPL treatments, showed significant quantitative increases in both collagen fibers (P < 0.05) and elastic fibers (P < 0.01). Collagen fibers demonstrated a better parallel distribution in relation to the epidermis.
IPL treatment significantly increased the number of collagen and elastic fibers within the dermis and improved the parallel distribution of collagen fibers in relation to the epidermis. These results were evident after three IPL treatments. Lasers Surg. Med. © 2018 Wiley Periodicals, Inc.
人类皮肤老化包括自然老化和光老化，其特征是胶原蛋白减少和异常弹性纤维沉积。强脉冲光（IPL）光源在医学上被广泛用于治疗各种美容问题，包括光损伤皮肤。很少有研究探讨IPL产生的微观变化。本研究的目的是定量评估IPL对小鼠胶原蛋白和弹性纤维的影响。
40只雌性BALB/c小鼠分为四个亚组。第1组为对照组（n = 10），第2、3、4组为治疗组（每组n = 10）。第2组接受一次治疗，第3组接受两次治疗，第4组每2周接受三次治疗。在每只小鼠最后一次治疗后24小时从照射区域获取皮肤组织。使用苦味酸天狼星红法鉴定胶原纤维。用魏格特-臭氧染色标记弹性纤维。所有样本均通过光学显微镜使用分析系统图像进行分析和定量。
接受三次IPL治疗的第4组，胶原纤维（P < 0.05）和弹性纤维（P < 0.01）的数量均显著增加。胶原纤维相对于表皮呈现出更好的平行分布。
IPL治疗显著增加了真皮内胶原纤维和弹性纤维的数量，并改善了胶原纤维相对于表皮的平行分布。这些结果在三次IPL治疗后很明显。激光外科与医学。©2018威利期刊公司。
